Endothelin-1 and its receptors on haemorrhoidal tissue: a potential site for therapeutic intervention

Lohsiriwat, Varut; Scholefield, John H.; Wilson, Vincent G.; Dashwood, Michael R.

Abstract

© 2017 The British Pharmacological Society Background and Purpose: Haemorrhoids is a common anorectal condition affecting millions worldwide. We have studied the effect of endothelin-1 (ET-1) and the role of endothelin ETA and ETB receptors in haemorrhoid tissue. Experimental Approach: Protein expression of ET-1, ETA and ETB receptors were compared between haemorrhoids and normal rectal submucosa using Western blot analysis, with the localization of proteins determined by autoradiography and immunohistochemistry. Effects of ET-1 and sarafotoxin 6a on human colonic and rectal arteries and veins was assessed by wire myography and the involvement of receptor subtypes established by selective antagonists. Key Results: Dense binding of [125I]-ET-1 to haemorrhoidal sections was reduced by selective receptor antagonists. A higher density of ETB than ETA receptors was found in haemorrhoidal, than in control rectal tissue and confirmed by Western blot analysis. ETA and ETB receptors were localized to smooth muscle of haemorrhoidal arteries and veins, with ETB receptors on the endothelium. Human colonic and rectal arteries and veins were similarly sensitive to ET-1 and affected by the ETA selective antagonist, but sarafotoxin S6a-induced contractions were more pronounced in veins and antagonized by a selective ETB receptor antagonist. Conclusions and Implications: ETA and ETB receptors are present in human haemorrhoids with ETB receptors predominating. ETA receptors are activated by ET-1 to mediate a contraction in arteries and veins, but the latter are selectively activated by sarafotoxin S6a – a response that involves ETB receptors at low concentrations. Selective ETB agonists may have therapeutic potential to reduce congestion of the haemorrhoidal venous sinusoids.

Citation

Lohsiriwat, V., Scholefield, J. H., Wilson, V. G., & Dashwood, M. R. (2017). Endothelin-1 and its receptors on haemorrhoidal tissue: a potential site for therapeutic intervention. British Journal of Pharmacology, 174(7), 569-579. https://doi.org/10.1111/bph.13719

• Journal Article Type: Article
• Acceptance Date: Jul 10, 2016
• Online Publication Date: Feb 16, 2017
• Publication Date: 2017-04
• Deposit Date: Jan 22, 2021
• Journal: British Journal of Pharmacology
• Print ISSN: 0007-1188
• Electronic ISSN: 1476-5381
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 174
• Issue: 7
• Pages: 569-579
• DOI: https://doi.org/10.1111/bph.13719
• Public URL: https://nottingham-repository.worktribe.com/output/3139649
• Publisher URL: https://bpspubs.onlinelibrary.wiley.com/doi/full/10.1111/bph.13719