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The prognostic significance of Cathepsin V (CTSV/CTSL2) in breast ductal carcinoma in situ

Toss, Michael S.; Miligy, Islam M.; Gorringe, Kylie L.; Mittal, Karuna; Green, Andrew R.; Aneja, Ritu; Ellis, Ian O.; Rakha, Emad A.

Authors

Kylie L. Gorringe

Karuna Mittal

Andrew R. Green

Ritu Aneja

Emad A. Rakha emad.rakha@nuh.nhs.uk



Abstract

Aims: Cathepsin V (CTSV/CTSL2) is a lysosomal cystine proteinase and plays a role in extracellular matrix degradation. It is associated with poor prognosis in invasive breast cancer (IBC), but its role in breast ductal carcinoma in situ (DCIS) remains unclear. In this study, we aimed to evaluate the prognostic significance of CTSV in DCIS.
Methods: CTSV protein expression was immunohistochemically assessed in a well characterised and annotated cohort of DCIS comprising, pure DCIS (n=776) and DCIS coexisting with IBC (n=239). CTSV expression was analysed in tumour cells and surrounding stroma, including its association with clinicopathological parameters and outcome.
Results: In pure DCIS, high CTSV expression was observed in 29% of epithelial tumour cells and 20% of surrounding stroma. High expression in both components was associated with features of poor prognosis including higher nuclear grade, hormone receptor negativity and HER2 positivity. In addition, stromal CTSV expression was associated with larger DCIS size, comedo type necrosis and high proliferation index. DCIS associated with IBC showed higher CTSV expression than pure DCIS either within the epithelial tumour cells or surrounding stroma (p

Journal Article Type Article
Publication Date Aug 23, 2019
Print ISSN 0021-9746
Publisher BMJ Publishing Group
Peer Reviewed Peer Reviewed
Institution Citation Toss, M. S., Miligy, I. M., Gorringe, K. L., Mittal, K., Green, A. R., Aneja, R., …Rakha, E. A. (2019). The prognostic significance of Cathepsin V (CTSV/CTSL2) in breast ductal carcinoma in situ. Journal of Clinical Pathology, doi:10.1136/jclinpath-2019-205939
DOI https://doi.org/10.1136/jclinpath-2019-205939
Keywords DCIS; proteolysis; CTSV; stroma; recurrence
Publisher URL https://jcp.bmj.com/content/early/2019/08/22/jclinpath-2019-205939
Additional Information This article has been accepted for publication in Journal of Clinical Pathology following peer review, and the Version of Record can be accessed online at http://dx.doi.org/10.1136/jclinpath-2019-205939 © Authors (or their employer(s))