Jun Ma
Metabolomic signature of mouse cerebral cortex following Toxoplasma gondii infection
Ma, Jun; He, Jun-Jun; Hou, Jun-Ling; Zhou, Chun-Xue; Zhang, Fu-Kai; Elsheikha, Hany M.; Zhu, Xing-Quan
Authors
Jun-Jun He
Jun-Ling Hou
Chun-Xue Zhou
Fu-Kai Zhang
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Xing-Quan Zhu
Abstract
Background: The protozoan parasite Toxoplasma gondii infects and alters the neurotransmission in cerebral cortex and other brain regions, leading to neurobehavioral and neuropathologic changes in humans and animals. However, the molecules that contribute to these changes remain largely unknown.
Methods: We have investigated the impact of T. gondii infection on the overall metabolism of mouse cerebral cortex. Mass-spectrometry-based metabolomics and multivariate statistical analysis were employed to discover metabolomic signatures that discriminate between cerebral cortex of T. gondii-infected and uninfected control mice.
Results: Our results identified 73, 67 and 276 differentially abundant metabolites, which were involved in 25, 37 and 64 pathways at 7, 14 and 21 days post-infection (dpi), respectively. Metabolites in the unsaturated fatty acid biosynthesis pathway were upregulated as the infection progressed, indicating that T. gondii induces the biosynthesis of unsaturated fatty acids to promote its own growth and survival. Some of the downregulated metabolites were related to pathways, such as steroid hormone biosynthesis and arachidonic acid metabolism. Nine metabolites were identified as T. gondii responsive metabolites, namely galactosylsphingosine, arachidonic acid, LysoSM(d18:1), l-palmitoylcarnitine, calcitetrol, 27-Deoxy-5b-cyprinol, l-homophenylalanine, oleic acid and ceramide (d18:1/16:0).
Conclusions: Our data provide novel insight into the dysregulation of the metabolism of the mouse cerebral cortex during T. gondii infection and have important implications for studies of T. gondii pathogenesis.
Citation
Ma, J., He, J., Hou, J., Zhou, C., Zhang, F., Elsheikha, H. M., & Zhu, X. (2019). Metabolomic signature of mouse cerebral cortex following Toxoplasma gondii infection. Parasites and Vectors, 12, 1-11. https://doi.org/10.1186/s13071-019-3623-4
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 19, 2019 |
Online Publication Date | Jul 29, 2019 |
Publication Date | Jul 29, 2019 |
Deposit Date | Aug 1, 2019 |
Journal | Parasites & Vectors |
Electronic ISSN | 1756-3305 |
Publisher | Springer Nature |
Peer Reviewed | Peer Reviewed |
Volume | 12 |
Article Number | 373 |
Pages | 1-11 |
DOI | https://doi.org/10.1186/s13071-019-3623-4 |
Keywords | Metabolomics, Neuropathy, Cerebral cortex, Toxoplasma gondii, Host-parasite interaction, Metabolism |
Public URL | https://nottingham-repository.worktribe.com/output/2371668 |
Publisher URL | https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-019-3623-4 |
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