Ex-vivo Raman spectroscopy mapping of lung tissue: label-free molecular characterisation of non-tumorous and cancerous tissues

Bourbousson, Manon; Soomro, Irshad; Baldwin, David; Notingher, Ioan

Abstract

Raman spectroscopy mapping was used to study ex vivo fresh lung tissues and compare to histology sections. The Raman mapping measurements revealed differences in the molecular composition of normal lung tissue, adenocarcinoma, and squamous cell carcinoma (SCC). Molecular heterogeneity of the tissue samples was well captured by the k-means clustering analysis of the Raman datasets, as confirmed by the correlation with the adjacent haematoxylin and eosin (H&E) stained tissue sections. The results indicate that the fluorescence background varies considerably even in samples that appear structurally uniform in the H&E images, both for normal and tumor tissue. The results show that characteristic Raman bands can be used to discriminate between tumorous and nontumorous lung tissues and between adenocarcinoma and SCC tissues. These results indicate the potential to develop Raman classifications models for lung tissues based on the Raman spectral differences at the microscopic level, which can be used for tissue diagnosis or treatment stratification.

Citation

Bourbousson, M., Soomro, I., Baldwin, D., & Notingher, I. (2019). Ex-vivo Raman spectroscopy mapping of lung tissue: label-free molecular characterisation of non-tumorous and cancerous tissues. Journal of Medical Imaging, 6(3), Article 036001. https://doi.org/10.1117/1.JMI.6.3.036001

• Journal Article Type: Article
• Acceptance Date: Jul 16, 2019
• Online Publication Date: Aug 9, 2019
• Publication Date: Aug 9, 2019
• Deposit Date: Jul 31, 2019
• Publicly Available Date: Jul 31, 2019
• Journal: Journal of Medical Imaging
• Electronic ISSN: 2329-4310
• Publisher: Society of Photo-optical Instrumentation Engineers
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 3
• Article Number: 036001
• DOI: https://doi.org/10.1117/1.JMI.6.3.036001
• Keywords: Raman spectroscopy, Mapping, Lung cancer, Diagnosis
• Public URL: https://nottingham-repository.worktribe.com/output/2361256
• Publisher URL: https://www.spiedigitallibrary.org/journals/journal-of-medical-imaging/volume-6/issue-03/036001/Ex-vivo-Raman-spectroscopy-mapping-of-lung-tissue--label/10.1117/1.JMI.6.3.036001.full