The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases

Pander, Bart; Harris, Gemma; Scott, David J.; Winzer, Klaus; Köpke, Michael; Simpson, Sean D.; Minton, Nigel P.; Henstra, Anne M.

Abstract

Carbonic anhydrase catalyses the interconversion of carbon dioxide and water to bicarbonate and protons. It was unknown if the industrial relevant acetogen Clostridium autoethanogenum possesses these enzymes. We identified two putative carbonic anhydrase genes in its genome, one of the β class and one of the γ class. Carbonic anhydrase activity was found for the purified β class enzyme, but not the γ class candidate. Functional complementation of an Escherichia coli carbonic anhydrase knock-out mutant showed that the β class carbonic anhydrase could complement this activity, but not the γ class candidate gene. Phylogenetic analysis showed that the β class carbonic anhydrase of Clostridium autoethanogenum represents a novel sub-class of β class carbonic anhydrases that form the F-clade. The members of this clade have the shortest primary structure of any known carbonic anhydrase.

Citation

Pander, B., Harris, G., Scott, D. J., Winzer, K., Köpke, M., Simpson, S. D., …Henstra, A. M. (2019). The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases. Applied Microbiology and Biotechnology, 103(17), 7275-7286. https://doi.org/10.1007/s00253-019-10015-w

• Journal Article Type: Article
• Acceptance Date: Jul 11, 2019
• Online Publication Date: Jul 25, 2019
• Publication Date: 2019-09
• Deposit Date: Jul 30, 2019
• Publicly Available Date: Jul 30, 2019
• Journal: Applied Microbiology and Biotechnology
• Print ISSN: 0175-7598
• Electronic ISSN: 1432-0614
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 103
• Issue: 17
• Pages: 7275-7286
• DOI: https://doi.org/10.1007/s00253-019-10015-w
• Keywords: Biotechnology; Applied Microbiology and Biotechnology; General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/2357174
• Publisher URL: https://link.springer.com/article/10.1007%2Fs00253-019-10015-w
• Additional Information: Received: 14 May 2019; Revised: 5 July 2019; Accepted: 8 July 2019; First Online: 25 July 2019; : ; : BP declares that she has no conflict of interest.GH declares that he has no conflict of interest.DJS declares that he has no conflict of interest.KW declares that he has no conflict of interest.MK is employed by LanzaTech. LanzaTech has commercial interest in gas fermentation.SDS is employed by LanzaTech. LanzaTech has commercial interest in gas fermentation.NPM declares that he has no conflict of interest.AMH declares that he has no conflict of interest.; : This article does not contain any studies with human participants or animals performed by any of the authors.