Skip to main content

Research Repository

See what's under the surface

Advanced Search

The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases

Pander, Bart; Harris, Gemma; Scott, David J.; Winzer, Klaus; Köpke, Michael; Simpson, Sean D.; Minton, Nigel P.; Henstra, Anne M.

Authors

Bart Pander

Gemma Harris

David J. Scott

Klaus Winzer

Michael Köpke

Sean D. Simpson

Nigel P. Minton

Anne M. Henstra



Abstract

Carbonic anhydrase catalyses the interconversion of carbon dioxide and water to bicarbonate and protons. It was unknown if the industrial relevant acetogen Clostridium autoethanogenum possesses these enzymes. We identified two putative carbonic anhydrase genes in its genome, one of the β class and one of the γ class. Carbonic anhydrase activity was found for the purified β class enzyme, but not the γ class candidate. Functional complementation of an Escherichia coli carbonic anhydrase knock-out mutant showed that the β class carbonic anhydrase could complement this activity, but not the γ class candidate gene. Phylogenetic analysis showed that the β class carbonic anhydrase of Clostridium autoethanogenum represents a novel sub-class of β class carbonic anhydrases that form the F-clade. The members of this clade have the shortest primary structure of any known carbonic anhydrase.

Journal Article Type Article
Publication Date 2019-09
Journal Applied Microbiology and Biotechnology
Print ISSN 0175-7598
Electronic ISSN 1432-0614
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 103
Issue 17
Pages 7275-7286
APA6 Citation Pander, B., Harris, G., Scott, D. J., Winzer, K., Köpke, M., Simpson, S. D., …Henstra, A. M. (2019). The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases. Applied Microbiology and Biotechnology, 103(17), 7275-7286. doi:10.1007/s00253-019-10015-w
DOI https://doi.org/10.1007/s00253-019-10015-w
Keywords Biotechnology; Applied Microbiology and Biotechnology; General Medicine
Publisher URL https://link.springer.com/article/10.1007%2Fs00253-019-10015-w
Additional Information Received: 14 May 2019; Revised: 5 July 2019; Accepted: 8 July 2019; First Online: 25 July 2019; : ; : BP declares that she has no conflict of interest.GH declares that he has no conflict of interest.DJS declares that he has no conflict of interest.KW declares that he has no conflict of interest.MK is employed by LanzaTech. LanzaTech has commercial interest in gas fermentation.SDS is employed by LanzaTech. LanzaTech has commercial interest in gas fermentation.NPM declares that he has no conflict of interest.AMH declares that he has no conflict of interest.; : This article does not contain any studies with human participants or animals performed by any of the authors.

Files

The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases (1 Mb)
PDF





You might also like



Downloadable Citations

;