Assessment of rapeseed oil body (oleosome) lipolytic activity as an effective predictor of emulsion purity and stability

De Chirico, Simone; di Bari, Vincenzo; Romero Guzm�n, Mar�a Juliana; Nikiforidis, Costas; Foster, Tim; Gray, David

Abstract

© 2020 Elsevier Ltd The lipolytic activity in oil body creams as affected by recovery and washing protocols was investigated. The effect of thermal treatment on the hydrolytic activity and physical stability of fresh and aged (up to 30 days) oil body emulsions was studied. The use of alkaline pH solutions (9.5) to soak and grind rapeseeds were more effective reducing the contamination of oil body material from seed proteins/enzymes, compared with neutral pHs. Soaking and grinding seeds with a NaHCO3 solution (0.1 M, pH 9.5) yielded oil bodies with a similar composition to those prepared in urea (9 M); however, the physical stability over storage was compromised due to the presence of hydrolytic enzymes. Heating a dispersion of oil bodies for 6 mins at 95 °C did not alter the physical properties of oil bodies and significantly reduced lipolytic activity (>90% enzyme inactivation), resulting in a stable emulsion.

Citation

De Chirico, S., di Bari, V., Romero Guzmán, M. J., Nikiforidis, C., Foster, T., & Gray, D. (2020). Assessment of rapeseed oil body (oleosome) lipolytic activity as an effective predictor of emulsion purity and stability. Food Chemistry, 316, Article 126355. https://doi.org/10.1016/j.foodchem.2020.126355

• Journal Article Type: Article
• Acceptance Date: Feb 3, 2020
• Online Publication Date: Feb 4, 2020
• Publication Date: Jun 30, 2020
• Deposit Date: Jul 15, 2019
• Publicly Available Date: Feb 5, 2021
• Journal: Food Chemistry
• Print ISSN: 0308-8146
• Electronic ISSN: 1873-7072
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 316
• Article Number: 126355
• DOI: https://doi.org/10.1016/j.foodchem.2020.126355
• Keywords: oil bodies; lipolytic activity; purity; stability; rapeseed
• Public URL: https://nottingham-repository.worktribe.com/output/2308696
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0308814620302090?via%3Dihub