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A low-passage insect-cell isolate of bluetongue virus uses a macropinocytosis-like entry pathway to infect natural target cells derived from the bovine host

Stevens, Lisa M.; Moffat, Katy; Cooke, Lyndsay; Nomikou, Kyriaki; Mertens, Peter P. C.; Jackson, Terry; Darpel, Karin E.

A low-passage insect-cell isolate of bluetongue virus uses a macropinocytosis-like entry pathway to infect natural target cells derived from the bovine host Thumbnail


Authors

Lisa M. Stevens

Katy Moffat

Lyndsay Cooke

Kyriaki Nomikou

Terry Jackson

Karin E. Darpel



Abstract

Bluetongue virus (BTV) causes an economically important disease in domestic and wildlife ruminants and is transmitted by Culicoides biting midges. In ruminants, BTV has a wide cell tropism that includes endothelial cells of vascular and lymphatic vessels as important cell targets for virus replication, and several cell types of the immune system including monocytes, macrophages and dendritic cells. Thus, cell-entry represents a particular challenge for BTV as it infects many different cell types in widely diverse vertebrate and invertebrate hosts. Improved understanding of BTV cell-entry could lead to novel antiviral approaches that can block virus transmission from cell to cell between its invertebrate and vertebrate hosts. Here, we have investigated BTV cell-entry using endothelial cells derived from the natural bovine host (BFA cells) and purified whole virus particles of a low-passage, insect-cell isolate of a virulent strain of BTV-1. Our results show that the main entry pathway for infection of BFA cells is dependent on actin and dynamin, and shares certain characteristics with macropinocytosis. The ability to use a macropinocytosis-like entry route could explain the diverse cell tropism of BTV and contribute to the efficiency of transmission between vertebrate and invertebrate hosts.

Citation

Stevens, L. M., Moffat, K., Cooke, L., Nomikou, K., Mertens, P. P. C., Jackson, T., & Darpel, K. E. (2019). A low-passage insect-cell isolate of bluetongue virus uses a macropinocytosis-like entry pathway to infect natural target cells derived from the bovine host. Journal of General Virology, 100(4), 568-582. https://doi.org/10.1099/jgv.0.001240

Journal Article Type Article
Acceptance Date Feb 12, 2019
Online Publication Date Mar 7, 2019
Publication Date Apr 1, 2019
Deposit Date May 10, 2019
Publicly Available Date Mar 29, 2024
Journal Journal of General Virology
Print ISSN 0022-1317
Electronic ISSN 1465-2099
Publisher Microbiology Society
Peer Reviewed Peer Reviewed
Volume 100
Issue 4
Pages 568-582
DOI https://doi.org/10.1099/jgv.0.001240
Keywords Endocytosis; Macropinocytosis; Bluetongue virus; Cell-entry; Virus; Bovine; Endothelial cells
Public URL https://nottingham-repository.worktribe.com/output/2038043
Publisher URL https://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001240

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