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Slow cycling intestinal stem cell and Paneth cell responses to Trichinella spiralis infection

Javkar, Tanvi; Hughes, Kevin R.; Sablitzky, Fred; Behnke, Jerzy M.; Mahida, Yashwant R.

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Authors

Tanvi Javkar

Kevin R. Hughes

Fred Sablitzky

Jerzy M. Behnke

YASH MAHIDA yash.mahida@nottingham.ac.uk
Professor of Medicine



Abstract

There is limited information regarding responses by slow cycling stem cells during T. spiralis-induced T-cell mediated intestinal inflammation and how such responses may relate to those of Paneth cells. Transgenic mice, in which doxycycline induces expression of histone 2B (H2B)-green fluorescent protein (GFP), were used. Following discontinuation of doxycycline (“chase” period), retention of H2B-GFP enabled the identification of slow cycling stem cells and long-lived Paneth cells. Inflammation in the small intestine (SI) was induced by oral administration of T. spiralis muscle larvae. Epithelial retention of H2B-GFP per crypt cell position (cp) was studied following immunohistochemistry and using the Score and Wincrypts program. Compared to non-infected controls, there was significant reduction in the number of H2B-GFP-retaining stem cells in T. spiralis-infected small intestines. H2B-GFP-retaining stem cells peaked at around cp 4 in control sections, but smaller peaks at higher cell positions (>10) were seen in sections of inflamed small intestines. In the latter, there was a significant increase in the total number of Paneth cells, with significant reduction in H2B-GFP-retaining Paneth cells, but a marked increase in unlabelled (H2B-GFP-negative) Paneth cells. In conclusion, following T. spiralis-infection, putative slow cycling stem cell numbers were reduced. A marked increase in newly generated Paneth cells at the crypt base led to higher cell positions of the remaining slow cycling stem cells.

Citation

Javkar, T., Hughes, K. R., Sablitzky, F., Behnke, J. M., & Mahida, Y. R. (2020). Slow cycling intestinal stem cell and Paneth cell responses to Trichinella spiralis infection. Parasitology International, 74, Article 101923. https://doi.org/10.1016/j.parint.2019.05.001

Journal Article Type Article
Acceptance Date May 1, 2019
Online Publication Date May 2, 2019
Publication Date 2020-02
Deposit Date May 10, 2019
Publicly Available Date Mar 28, 2024
Journal Parasitology International
Print ISSN 1383-5769
Electronic ISSN 1873-0329
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 74
Article Number 101923
DOI https://doi.org/10.1016/j.parint.2019.05.001
Keywords Stem cells; Paneth cells; Trichinella spiralis
Public URL https://nottingham-repository.worktribe.com/output/2037955
Publisher URL https://www.sciencedirect.com/science/article/pii/S1383576918304215?via%3Dihub

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