Wei Wang
Unravelling Strain-Specific Modifications of Toxoplasma gondii tRNA and sncRNA Using LC-MS/MS
Wang, Wei; Yang, Yi; Guo, Huanping; Li, Ming Han; Chen, Xiao Qing; Wei, Xin Yu; Chen, Yu; Elsheikha, Hany M.; Zhang, Xiao Xuan
Authors
Yi Yang
Huanping Guo
Ming Han Li
Xiao Qing Chen
Xin Yu Wei
Yu Chen
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Xiao Xuan Zhang
Contributors
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Researcher
Abstract
Many RNA modifications have been detected in rRNA, tRNA and small noncoding RNA (sncRNA) as well as in low-abundance RNA species such mRNA. Although RNA modifications play roles in many cellular and biological processes in various domains of life, knowledge about the diversity and role of RNA modifications in Toxoplasma gondii is limited. In this study, RNA modifications in three T. gondii strains (RH type I, PRU type II, and VEG type III) with distinct virulence abilities were determined by liquid chromatography-tandem mass spectrometry. We compared the levels of modifications of four nucleotides in tRNA and sncRNA, characterized RNA modification patterns of different T. gondii strains, and determined the diversity of RNA modifications. We detected and quantified 22 modified nucleosides in both tRNA and sncRNA. Significant differences in the diversity of the modified nucleosides were found between the three T. gondii strains. RNA modifications were correlated with the expression of many T. gondii virulence proteins. Some of the identified modifications (e.g., 2'-O-methylinosine, pseudouridine) play a role in mediating the host-parasite interaction. These results provide novel insight into the global modifications in tRNA and sncRNA, and the diversity of RNA modifications between T. gondii strains with different virulence backgrounds. IMPORTANCE Although RNA modifications play roles in many cellular and developmental processes in various domains of life, knowledge about the patterns and functions of RNA modifications in T. gondii is limited. Here, a quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach was used to study global RNA modifications in T. gondii strains of distinct virulence backgrounds. We quantified 22 modified nucleosides in both tRNA and sncRNA. Significant T. gondii strain-specific differences in RNA modifications were detected. More tRNA modifications correlated with T. gondii virulence proteins than sncRNA modifications. RNA modifications were significantly correlated with virulence proteins. Our data provide the first comprehensive profiling of the modifications tRNA and sncRNA in T. gondii, expanding the diversity of RNA modifications in this parasite and suggesting new regulators for modulating its virulence.
Citation
Wang, W., Yang, Y., Guo, H., Li, M. H., Chen, X. Q., Wei, X. Y., …Zhang, X. X. (2023). Unravelling Strain-Specific Modifications of Toxoplasma gondii tRNA and sncRNA Using LC-MS/MS. Microbiology Spectrum, 11(3), Article e0356422. https://doi.org/10.1128/spectrum.03564-22
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 21, 2023 |
Online Publication Date | Apr 10, 2023 |
Publication Date | Apr 10, 2023 |
Deposit Date | Apr 10, 2023 |
Publicly Available Date | Mar 29, 2024 |
Journal | Microbiology Spectrum |
Electronic ISSN | 2165-0497 |
Peer Reviewed | Peer Reviewed |
Volume | 11 |
Issue | 3 |
Article Number | e0356422 |
DOI | https://doi.org/10.1128/spectrum.03564-22 |
Keywords | Infectious Diseases; Cell Biology; Microbiology (medical); Genetics; General Immunology and Microbiology; Ecology; Physiology |
Public URL | https://nottingham-repository.worktribe.com/output/19456357 |
Publisher URL | https://journals.asm.org/doi/10.1128/spectrum.03564-22 |
Files
Spectrum.03564-22
(2.4 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
You might also like
Companion animal parasites: 10 years on
(2023)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: digital-library-support@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search