Complement Factor H Mutation W1206R Causes Retinal Thrombosis and Ischemic Retinopathy in Mice

Song, Delu; Ueda, Yoshiyasu; Bhuyan, Rupak; Mohammed, Imran; Miwa, Takashi; Gullipali, Damodar; Kim, Hangsoo; Zhou, Lin; Song, Ying; Schultz, Hannah; Bargoud, Albert; Dunaief, Joshua L.; Song, Wen-Chao

Abstract

© 2019 American Society for Investigative Pathology Single-nucleotide polymorphisms and rare mutations in factor H (FH; official name, CFH) are associated with age-related macular degeneration and atypical hemolytic uremic syndrome, a form of thrombotic microangiopathy. Mice with the FH W1206R mutation (FH R/R ) share features with human atypical hemolytic uremic syndrome. Herein, we report that FH R/R mice exhibited retinal vascular occlusion and ischemia. Retinal fluorescein angiography demonstrated delayed perfusion and vascular leakage in FH R/R mice. Optical coherence tomography imaging of FH R/R mice showed retinal degeneration, edema, and detachment. Histologic analysis of FH R/R mice revealed retinal thinning, vessel occlusion, as well as degeneration of photoreceptors and retinal pigment epithelium. Immunofluorescence showed albumin leakage from blood vessels into the neural retina, and electron microscopy demonstrated vascular endothelial cell irregularity with narrowing of retinal and choroidal vessels. Knockout of C6, a component of the membrane attack complex, prevented the aforementioned retinal phenotype in FH R/R mice, consistent with membrane attack complex–mediated pathogenesis. Pharmacologic blockade of C5 also rescued retinas of FH R/R mice. This FH R/R mouse strain represents a model for retinal vascular occlusive disorders and ischemic retinopathy. The results suggest complement dysregulation can contribute to retinal vascular occlusion and that an anti-C5 antibody might be helpful for C5-mediated thrombotic retinal diseases.

Citation

Song, D., Ueda, Y., Bhuyan, R., Mohammed, I., Miwa, T., Gullipali, D., …Song, W. (2019). Complement Factor H Mutation W1206R Causes Retinal Thrombosis and Ischemic Retinopathy in Mice. American Journal of Pathology, 189(4), 826-838. https://doi.org/10.1016/j.ajpath.2019.01.009

• Journal Article Type: Article
• Acceptance Date: Jan 2, 2019
• Online Publication Date: Feb 1, 2019
• Publication Date: 2019-04
• Deposit Date: Jun 10, 2019
• Publicly Available Date: Feb 2, 2020
• Journal: American Journal of Pathology
• Print ISSN: 0002-9440
• Electronic ISSN: 1525-2191
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 189
• Issue: 4
• Pages: 826-838
• DOI: https://doi.org/10.1016/j.ajpath.2019.01.009
• Keywords: Pathology and Forensic Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/1860474
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0002944018306242?via%3Dihub