EXPANSIN A1-mediated radial swelling of pericycle cells positions anticlinal cell divisions during lateral root initiation

Ramakrishna, Priya; Ruiz Duarte, Paola; Rance, Graham A.; Schubert, Martin; Vordermaier, Vera; Vu, Lam Dai; Murphy, Evan; Vilches Barro, Amaya; Swarup, Kamal; Moirangthem, Kamaljit; Jørgensen, Bodil; van de Cotte, Brigitte; Goh, Tatsuaki; Lin, Zhefeng; Voβ, Ute; Beeckman, Tom; Bennett, Malcolm J.; Gevaert, Kris; Maizel, Alexis; De Smet, Ive

Abstract

In plants, postembryonic formation of new organs helps shape the adult organism. This requires the tight regulation of when and where a new organ is formed and a coordination of the underlying cell divisions. To build a root system, new lateral roots are continuously developing, and this process requires the tight coordination of asymmetric cell division in adjacent pericycle cells. We identified EXPANSIN A1 (EXPA1) as a cell wall modifying enzyme controlling the divisions marking lateral root initiation. Loss of EXPA1 leads to defects in the first asymmetric pericycle cell divisions and the radial swelling of the pericycle during auxin-driven lateral root formation. We conclude that a localized radial expansion of adjacent pericycle cells is required to position the asymmetric cell divisions and generate a core of small daughter cells, which is a prerequisite for lateral root organogenesis.

Citation

Ramakrishna, P., Ruiz Duarte, P., Rance, G. A., Schubert, M., Vordermaier, V., Vu, L. D., …De Smet, I. (2019). EXPANSIN A1-mediated radial swelling of pericycle cells positions anticlinal cell divisions during lateral root initiation. Proceedings of the National Academy of Sciences, 116(17), 8597-8602. https://doi.org/10.1073/pnas.1820882116

• Journal Article Type: Article
• Acceptance Date: Mar 6, 2019
• Online Publication Date: Apr 3, 2019
• Publication Date: Apr 23, 2019
• Deposit Date: Jun 11, 2019
• Publicly Available Date: Jun 17, 2019
• Journal: Proceedings of the National Academy of Sciences
• Print ISSN: 0027-8424
• Electronic ISSN: 1091-6490
• Publisher: National Academy of Sciences
• Peer Reviewed: Peer Reviewed
• Volume: 116
• Issue: 17
• Pages: 8597-8602
• DOI: https://doi.org/10.1073/pnas.1820882116
• Public URL: https://nottingham-repository.worktribe.com/output/1854538
• Publisher URL: https://www.pnas.org/content/116/17/8597