Development and evaluation of hotshot protocols for cost-and time-effective extraction of PCR-ready DNA from single freshwater mussel larvae (Bivalvia: Unionida)

Zieritz, Alexandra; Yasaeng, Pathamawadi; Razak, Nabilah Fatin Abd; Hongtrakul, Vipa; Kovitvadhi, Uthaiwan; Kanchanaketu, Thiti

Abstract

Freshwater mussels (Unionida) are unique in that their larvae (glochidium, haustorium or lasidium) parasitize vertebrate hosts, with a given mussel species metamorphosing only on a limited array of host species (Wächtler, Mansur & Richter, 2001). Collecting data on host fish identities is important for both conservation and commercial applications, but requires identification of single larvae to species level. This is most efficiently achieved by molecular species identification (White, McPheron & Stauffer, 1996; Kneeland & Rhymer, 2007, 2008; Boyer et al., 2011; Zieritz et al., 2012; Vannarattanarat et al., 2014), because morphological identification of larval mussels is time-consuming and sometimes unreliable (Wiles, 1975; O’brien, Williams & Hoggarth, 2003).

Citation

Zieritz, A., Yasaeng, P., Razak, N. F. A., Hongtrakul, V., Kovitvadhi, U., & Kanchanaketu, T. (2018). Development and evaluation of hotshot protocols for cost-and time-effective extraction of PCR-ready DNA from single freshwater mussel larvae (Bivalvia: Unionida). Journal of Molluscan Studies, 84(2), 198-201. https://doi.org/10.1093/mollus/eyy011

• Journal Article Type: Article
• Acceptance Date: Mar 15, 2018
• Online Publication Date: Apr 4, 2018
• Publication Date: May 1, 2018
• Deposit Date: Feb 14, 2019
• Journal: Journal of Molluscan Studies
• Print ISSN: 0260-1230
• Electronic ISSN: 1464-3766
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 84
• Issue: 2
• Pages: 198-201
• DOI: https://doi.org/10.1093/mollus/eyy011
• Public URL: https://nottingham-repository.worktribe.com/output/1548447
• Publisher URL: https://academic.oup.com/mollus/article/84/2/198/4961361