Ultrasound modulation of bioluminescence generated inside a turbid medium

Ahmad, Junaid; Jayet, Baptiste; Hill, Philip J.; Mather, Melissa L.; Dehghani, Hamid; Morgan, Stephen P.

Abstract

In vivo bioluminescence imaging (BLI) has poor spatial resolution owing to strong light scattering by tissue, which also affects quantitative accuracy. This paper proposes a hybrid acousto-optic imaging platform that images bioluminescence modulated at ultrasound (US) frequency inside an optically scattering medium. This produces an US modulated light within the tissue that reduces the effects of light scattering and improves the spatial resolution. The system consists of a continuously excited 3.5 MHz US transducer applied to a tissue like phantom of known optical properties embedded with bio-or chemiluminescent sources that are used to mimic in vivo experiments. Scanning US over the turbid medium modulates the luminescent sources deep inside tissue at several US scan points. These modulated signals are recorded by a photomultiplier tube and lock-in detection to generate a 1D profile. Indeed, high frequency US enables small focal volume to improve spatial resolution, but this leads to lower signal-to-noise ratio. First experimental results show that US enables localization of a small luminescent source (around 2 mm wide) deep (∼20 mm) inside a tissue phantom having a scattering coefficient of 80 cm-1. Two sources separated by 10 mm could be resolved 20 mm inside a chicken breast.

Citation

Ahmad, J., Jayet, B., Hill, P. J., Mather, M. L., Dehghani, H., & Morgan, S. P. (2017). Ultrasound modulation of bioluminescence generated inside a turbid medium. In A. A. Oraevsky, & L. V. Wang (Eds.), Proceedings SPIE 10064: Photons Plus Ultrasound: Imaging and Sensing 2017. https://doi.org/10.1117/12.2251349

• Conference Name: Photons Plus Ultrasound: Imaging and Sensing 2017
• Conference Location: San Francisco, California, United States
• Start Date: Jan 28, 2017
• End Date: Feb 2, 2017
• Acceptance Date: Jan 3, 2017
• Online Publication Date: Jan 29, 2017
• Publication Date: Mar 3, 2017
• Deposit Date: Jan 10, 2019
• Volume: 10064
• Book Title: Proceedings SPIE 10064: Photons Plus Ultrasound: Imaging and Sensing 2017
• DOI: https://doi.org/10.1117/12.2251349
• Public URL: https://nottingham-repository.worktribe.com/output/1463252
• Publisher URL: https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10064/1006454/Ultrasound-modulation-of-bioluminescence-generated-inside-a-turbid-medium/10.1117/12.2251349.short?SSO=1