Skip to main content

Research Repository

Advanced Search

IFN-? and IL-17A differentially influence the response of human macrophages and neutrophils to Pseudomonas aeruginosa infection


Sirina Muntaka

Yasir Almuhanna

Darryl Jackson

Research Development Manager

Afrakoma Afryie-Asante

Profile Image

Professor of Molecular Microbiology


Macrophages are important orchestrators of inflammation during bacterial infection acting both as effector cells and as regulators of neutrophil recruitment and life span. Differently activated macrophage populations with distinct inflammatory and microbicidal potential have been described. Our previous work unveiled a positive and a negative correlation between levels of IFN-? and IL-17A, respectively, and lung function in cystic fibrosis, particularly in patients chronically infected with P. aeruginosa. This study sought to define key parameters in human anti-bacterial immunity under Th1- and Th17-dominated inflammatory conditions; the final aim was to identify unique characteristics that could be fine-tuned therapeutically to minimise tissue damage while maximising bacterial clearance. Towards this aim neutrophils were incorporated into cultures of macrophages treated with IFN-? or IL-17A and infected with P. aeruginosa. The intent of this design was to model (i) initiation of inflammation by infected macrophages and (ii) delayed arrival of neutrophils and their exposure to macrophage-derived cytokines. Under these conditions IFN-? decreased bacterial killing and promoted production of the monocyte chemoattractant MCP-1. In contrast, IL-17A promoted bacterial killing but did not affect MCP-1 production. Secretion of the pyrogen IL-1? was significantly lower in the presence of IFN-? compared to IL-17A and correlated with levels of IL1B transcript in infected macrophages. These findings support the validity of this model to investigate human anti-bacterial immunity. Based on these observations, the protective and damaging roles of IFN-? and IL-17A, respectively, during P. aeruginosa infection could be caused by their contrasting effects on IL-1? and MCP-1 production.


Muntaka, S., Almuhanna, Y., Jackson, D., Singh, S., Afryie-Asante, A., Cámara, M., & Martínez-Pomares, L. (2019). IFN-γ and IL-17A differentially influence the response of human macrophages and neutrophils to Pseudomonas aeruginosa infection. Infection and Immunity, 87(2),

Journal Article Type Article
Acceptance Date Nov 7, 2018
Online Publication Date Nov 19, 2018
Publication Date Jan 14, 2019
Deposit Date Dec 18, 2018
Publicly Available Date Dec 18, 2018
Journal Infection and Immunity
Print ISSN 0019-9567
Electronic ISSN 1098-5522
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 87
Issue 2
Keywords gamma interferon; interleukin-1; interleukin-17; Pseudomonas aeruginosa; macrophages; neutrophils
Public URL
Publisher URL


You might also like

Downloadable Citations