Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses

Bartley, Kathryn; Deane, David; Percival, Ann; Dry, Inga R.; Grant, Dawn M.; Inglis, Neil F.; Mclean, Kevin; Manson, Erin D.T.; Imrie, Lisa H.J.; Haig, David M.; Lankester, Felix; Russell, George C.

Abstract

Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.

Citation

Bartley, K., Deane, D., Percival, A., Dry, I. R., Grant, D. M., Inglis, N. F., Mclean, K., Manson, E. D., Imrie, L. H., Haig, D. M., Lankester, F., & Russell, G. C. (2014). Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses. Veterinary Microbiology, 173(1-2), 17-26. https://doi.org/10.1016/j.vetmic.2014.07.004

• Journal Article Type: Article
• Acceptance Date: Jul 7, 2014
• Online Publication Date: Jul 15, 2014
• Publication Date: Sep 17, 2014
• Deposit Date: Dec 4, 2018
• Journal: Veterinary Microbiology
• Print ISSN: 0378-1135
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 173
• Issue: 1-2
• Pages: 17-26
• DOI: https://doi.org/10.1016/j.vetmic.2014.07.004
• Keywords: Inflammatory disease; Malignant catarrhal fever; Antibodies; Proteomics; Recombinant expression; Antigen identification
• Public URL: https://nottingham-repository.worktribe.com/output/1362437
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0378113514003277?via%3Dihub