Structure-Based Discovery of Novel Chemotypes for Adenosine A2A Receptor Antagonists

Katritch, Vsevolod; Jaakola, Veli-Pekka; Lane, J Robert; Lin, Judy; Ijzerman, Adriaan P; Yeager, Mark; Kufareva, Irina; Stevens, Raymond C; Abagyan, Ruben

Abstract

The recent progress in crystallography of G-protein coupled receptors opens an unprecedented venue for structure-based GPCR drug discovery. To test efficiency of the structure-based approach, we performed molecular docking and virtual ligand screening (VLS) of more than 4 million commercially available "drug-like" and ''lead-like'' compounds against the A(2A)AR 2.6 A resolution crystal structure. Out of 56 high ranking compounds tested in A(2A)AR binding assays, 23 showed affinities under 10 microM, 11 of those had sub-microM affinities and two compounds had affinities under 60 nM. The identified hits represent at least 9 different chemical scaffolds and are characterized by very high ligand efficiency (0.3-0.5 kcal/mol per heavy atom). Significant A(2A)AR antagonist activities were confirmed for 10 out of 13 ligands tested in functional assays. High success rate, novelty, and diversity of the chemical scaffolds and strong ligand efficiency of the A(2A)AR antagonists identified in this study suggest practical applicability of receptor-based VLS in GPCR drug discovery.

Citation

Katritch, V., Jaakola, V., Lane, J. R., Lin, J., Ijzerman, A. P., Yeager, M., …Abagyan, R. (2010). Structure-Based Discovery of Novel Chemotypes for Adenosine A2A Receptor Antagonists. Journal of Medicinal Chemistry, 53(4), 1799-1809. https://doi.org/10.1021/jm901647p

• Journal Article Type: Article
• Acceptance Date: Jan 22, 2010
• Publication Date: Feb 25, 2010
• Deposit Date: Apr 22, 2020
• Journal: Journal of Medicinal Chemistry
• Print ISSN: 0022-2623
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 53
• Issue: 4
• Pages: 1799-1809
• DOI: https://doi.org/10.1021/jm901647p
• Public URL: http://dx.doi.org/10.1021/jm901647p
• Publisher URL: https://pubs.acs.org/doi/10.1021/jm901647p