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In vivo Magnetic Resonance Imaging of Tumor Protease Activity

Haris, Mohammad; Singh, Anup; Mohammed, Imran; Ittyerah, Ranjit; Nath, Kavindra; Nanga, Ravi Prakash Reddy; Debrosse, Catherine; Kogan, Feliks; Cai, Kejia; Poptani, Harish; Reddy, Damodar; Hariharan, Hari; Reddy, Ravinder

Authors

Mohammad Haris

Anup Singh

Ranjit Ittyerah

Kavindra Nath

Ravi Prakash Reddy Nanga

Catherine Debrosse

Feliks Kogan

Kejia Cai

Harish Poptani

Damodar Reddy

Hari Hariharan

Ravinder Reddy



Abstract

Increased expression of cathepsins has diagnostic as well as prognostic value in several types of cancer. Here, we demonstrate a novel magnetic resonance imaging (MRI) method, which uses poly-L-glutamate (PLG) as an MRI probe to map cathepsin expression in vivo, in a rat brain tumor model. This noninvasive, high-resolution and non-radioactive method exploits the differences in the CEST signals of PLG in the native form and cathepsin mediated cleaved form. The method was validated in phantoms with known physiological concentrations, in tumor cells and in an animal model of brain tumor along with immunohistochemical analysis. Potential applications in tumor diagnosis and evaluation of therapeutic response are outlined.

Journal Article Type Article
Publication Date 2015-05
Journal Scientific Reports
Print ISSN 2045-2322
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 4
Issue 1
Article Number 6081
APA6 Citation Haris, M., Singh, A., Mohammed, I., Ittyerah, R., Nath, K., Nanga, R. P. R., …Reddy, R. (2015). In vivo Magnetic Resonance Imaging of Tumor Protease Activity. https://doi.org/10.1038/srep06081
DOI https://doi.org/10.1038/srep06081
Publisher URL https://www.nature.com/articles/srep06081

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