In vivo Magnetic Resonance Imaging of Tumor Protease Activity

Haris, Mohammad; Singh, Anup; Mohammed, Imran; Ittyerah, Ranjit; Nath, Kavindra; Nanga, Ravi Prakash Reddy; Debrosse, Catherine; Kogan, Feliks; Cai, Kejia; Poptani, Harish; Reddy, Damodar; Hariharan, Hari; Reddy, Ravinder

Abstract

Increased expression of cathepsins has diagnostic as well as prognostic value in several types of cancer. Here, we demonstrate a novel magnetic resonance imaging (MRI) method, which uses poly-L-glutamate (PLG) as an MRI probe to map cathepsin expression in vivo, in a rat brain tumor model. This noninvasive, high-resolution and non-radioactive method exploits the differences in the CEST signals of PLG in the native form and cathepsin mediated cleaved form. The method was validated in phantoms with known physiological concentrations, in tumor cells and in an animal model of brain tumor along with immunohistochemical analysis. Potential applications in tumor diagnosis and evaluation of therapeutic response are outlined.

Citation

Haris, M., Singh, A., Mohammed, I., Ittyerah, R., Nath, K., Nanga, R. P. R., …Reddy, R. (2015). In vivo Magnetic Resonance Imaging of Tumor Protease Activity. Scientific Reports, 4(1), Article 6081. https://doi.org/10.1038/srep06081

• Journal Article Type: Article
• Acceptance Date: Jul 24, 2014
• Online Publication Date: Aug 15, 2014
• Publication Date: 2015-05
• Deposit Date: Nov 16, 2018
• Publicly Available Date: Apr 12, 2019
• Journal: Scientific Reports
• Print ISSN: 2045-2322
• Publisher: Nature Research
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 1
• Article Number: 6081
• DOI: https://doi.org/10.1038/srep06081
• Public URL: https://nottingham-repository.worktribe.com/output/1277060
• Publisher URL: https://www.nature.com/articles/srep06081