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A modified high-throughput analysis of PLFAs in soil

Ellis, Stephanie; Ritz, Karl

Authors

Stephanie Ellis

Karl Ritz



Abstract

Microbial community profiling via phospholipid fatty-acid (PLFA) analysis is an insightful technique which elucidates the phenotypic structure of microbial assemblages within soil. Previous iterations of PLFA analysis have used large quantities of chemicals and can take extended periods of time to perform. Another barrier to the implementation of this method is the cost and availability of specialised machinery. We report on a high-throughput method which reduces both the time to extract PLFAs from soil and reduces the quantity of chemicals required.

Journal Article Type Article
Publication Date Oct 31, 2018
Journal MethodsX
Electronic ISSN 2215-0161
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 5
Pages 1491-1497
APA6 Citation Ellis, S., & Ritz, K. (2018). A modified high-throughput analysis of PLFAs in soil. MethodsX, 5, 1491-1497. https://doi.org/10.1016/j.mex.2018.10.022
DOI https://doi.org/10.1016/j.mex.2018.10.022
Keywords Phospholipid fatty acids; Microbial community; Microbial ecology; Fatty acids
Publisher URL https://www.sciencedirect.com/science/article/pii/S2215016118301791?via%3Dihub

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