A modified high-throughput analysis of PLFAs in soil

Ellis, Stephanie; Ritz, Karl

Abstract

Microbial community profiling via phospholipid fatty-acid (PLFA) analysis is an insightful technique which elucidates the phenotypic structure of microbial assemblages within soil. Previous iterations of PLFA analysis have used large quantities of chemicals and can take extended periods of time to perform. Another barrier to the implementation of this method is the cost and availability of specialised machinery. We report on a high-throughput method which reduces both the time to extract PLFAs from soil and reduces the quantity of chemicals required.

Citation

Ellis, S., & Ritz, K. (2018). A modified high-throughput analysis of PLFAs in soil. MethodsX, 5, 1491-1497. https://doi.org/10.1016/j.mex.2018.10.022

• Journal Article Type: Article
• Acceptance Date: Oct 22, 2018
• Online Publication Date: Oct 31, 2018
• Publication Date: Oct 31, 2018
• Deposit Date: Nov 12, 2018
• Publicly Available Date: Nov 29, 2018
• Journal: MethodsX
• Electronic ISSN: 2215-0161
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Pages: 1491-1497
• DOI: https://doi.org/10.1016/j.mex.2018.10.022
• Keywords: Phospholipid fatty acids; Microbial community; Microbial ecology; Fatty acids
• Public URL: https://nottingham-repository.worktribe.com/output/1242095
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S2215016118301791?via%3Dihub