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Isolation, culturing and 3D bioprinting equine myoblasts

Aimaletdinov, Alexander; Abyzova, Maria; Kurilov, Igor; Yuferova, Alina; Rutland, Catrin; Rizvanov, Albert; Zakirova, Elena


Alexander Aimaletdinov

Maria Abyzova

Igor Kurilov

Alina Yuferova

Albert Rizvanov

Elena Zakirova


Isolating and culturing myoblasts is essential for techniques such as tissue regeneration and in vitro meat production. This research describes a protocol to isolate primary myoblasts from skeletal muscle of an adult horse. The equine primary myoblasts expressed markers specific to myoblasts and had multipotent potential capabilities with differentiation into chondrocytes, adipocytes and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies showed that the use of GelMa bioink and ionic cross-linking did not have negative effects on cell proliferation at the beginning of cultivation. However, cells showed reduced proliferative activity by day 40 following in vitro culturing. The population of primary equine myoblasts obtained from an adult individual, and propagated on microcarriers and bioink, did not meet the requirements of the regenerative veterinary and manufacturing meat in vitro regarding the quantity and quality of the cells required. Nonetheless, further optimization of the cell scaling up process, including both microcarriers and/or the bioreactor program and bioprinting, is still important.


Aimaletdinov, A., Abyzova, M., Kurilov, I., Yuferova, A., Rutland, C., Rizvanov, A., & Zakirova, E. (2022). Isolation, culturing and 3D bioprinting equine myoblasts. Biological Communications, 67(3), 152-159.

Journal Article Type Article
Acceptance Date Jun 3, 2022
Online Publication Date Oct 10, 2022
Publication Date 2022-07
Deposit Date Oct 20, 2022
Publicly Available Date Oct 25, 2022
Journal Biological Communications
Print ISSN 2542-215
Electronic ISSN 2542-2154
Publisher St. Petersburg University Press
Peer Reviewed Peer Reviewed
Volume 67
Issue 3
Pages 152-159
Keywords Myoblasts, Bioprinting, Microcarriers
Public URL
Publisher URL


12821-Article Text-47902-1-10-20221009 Isolation Culturing And 3d Bioprinting Of Equine Myoblasts (2.9 Mb)

Copyright Statement
Copyright: © 2022 Aimaletdinov et al. This is an open-access article distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted <br /> distribution, and self-archiving free of charge.

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