Isolation, culturing and 3D bioprinting equine myoblasts

Aimaletdinov, Alexander; Abyzova, Maria; Kurilov, Igor; Yuferova, Alina; Rutland, Catrin; Rizvanov, Albert; Zakirova, Elena

doi:10.21638/spbu03.2022.302

Authors

Alexander Aimaletdinov

Maria Abyzova

Igor Kurilov

Alina Yuferova

CATRIN RUTLAND catrin.rutland@nottingham.ac.uk
Associate Professor

Albert Rizvanov

Elena Zakirova

Abstract

Isolating and culturing myoblasts is essential for techniques such as tissue regeneration and in vitro meat production. This research describes a protocol to isolate primary myoblasts from skeletal muscle of an adult horse. The equine primary myoblasts expressed markers specific to myoblasts and had multipotent potential capabilities with differentiation into chondrocytes, adipocytes and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies showed that the use of GelMa bioink and ionic cross-linking did not have negative effects on cell proliferation at the beginning of cultivation. However, cells showed reduced proliferative activity by day 40 following in vitro culturing. The population of primary equine myoblasts obtained from an adult individual, and propagated on microcarriers and bioink, did not meet the requirements of the regenerative veterinary and manufacturing meat in vitro regarding the quantity and quality of the cells required. Nonetheless, further optimization of the cell scaling up process, including both microcarriers and/or the bioreactor program and bioprinting, is still important.

Citation

Aimaletdinov, A., Abyzova, M., Kurilov, I., Yuferova, A., Rutland, C., Rizvanov, A., & Zakirova, E. (2022). Isolation, culturing and 3D bioprinting equine myoblasts. Biological Communications, 67(3), 152-159. https://doi.org/10.21638/spbu03.2022.302

Journal Article Type	Article
Acceptance Date	Jun 3, 2022
Online Publication Date	Oct 10, 2022
Publication Date	2022-07
Deposit Date	Oct 20, 2022
Publicly Available Date	Oct 25, 2022
Journal	Biological Communications
Print ISSN	2542-215
Electronic ISSN	2542-2154
Publisher	St. Petersburg University Press
Peer Reviewed	Peer Reviewed
Volume	67
Issue	3
Pages	152-159
DOI	https://doi.org/10.21638/spbu03.2022.302
Keywords	Myoblasts, Bioprinting, Microcarriers
Public URL	https://nottingham-repository.worktribe.com/output/12329504
Publisher URL	https://biocomm.spbu.ru/article/view/12821

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Copyright Statement
Copyright: © 2022 Aimaletdinov et al. This is an open-access article distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted <br /> distribution, and self-archiving free of charge.