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A regulatory module controlling GA-mediated endosperm cell expansion is critical for seed germination in Arabidopsis

S�nchez-Montesino, Roc�o; Bouza-Morcillo, Laura; Marquez, Julietta; Ghita, Melania; Duran-Nebreda, Salva; G�mez, Luis; Holdsworth, Michael J; Bassel, George; O�ate-S�nchez, Luis

A regulatory module controlling GA-mediated endosperm cell expansion is critical for seed germination in Arabidopsis Thumbnail


Authors

Roc�o S�nchez-Montesino

Laura Bouza-Morcillo

Julietta Marquez

Melania Ghita

Salva Duran-Nebreda

Luis G�mez

George Bassel

Luis O�ate-S�nchez



Abstract

A key component of seed germination is the interplay of mechanical forces governing embryo growth and the surrounding restraining endosperm tissue. Endosperm cell separation is therefore thought to play a critical role in the control of this developmental transition. Here we demonstrate that in Arabidopsis thaliana seeds, endosperm cell expansion is a key component of germination. Endosperm cells expand to accommodate embryo growth prior to germination. We show that this is an actively regulated process supported by spatiotemporal control of the cell expansion gene EXPANSIN 2 (EXPA2). The NAC transcription factors NAC25 and NAC1L were identified as upstream regulatory effectors of EXPA2 expression, GA-mediated endosperm expansion and seed germination. The DELLA protein RGL2 repressed activation of EXPA2 promoter by NAC. Our findings demonstrate a key role of this gene network in regulating endosperm cell-expansion to control the seed to seedling transition.

Citation

Sánchez-Montesino, R., Bouza-Morcillo, L., Marquez, J., Ghita, M., Duran-Nebreda, S., Gómez, L., …Oñate-Sánchez, L. (2019). A regulatory module controlling GA-mediated endosperm cell expansion is critical for seed germination in Arabidopsis. Molecular Plant, 12(1), 71-85. https://doi.org/10.1016/j.molp.2018.10.009

Journal Article Type Article
Acceptance Date Oct 31, 2018
Online Publication Date Nov 10, 2018
Publication Date Jan 7, 2019
Deposit Date Nov 6, 2018
Publicly Available Date Mar 29, 2024
Journal Molecular Plant
Print ISSN 1674-2052
Electronic ISSN 1752-9867
Publisher Oxford University Press (OUP)
Peer Reviewed Peer Reviewed
Volume 12
Issue 1
Pages 71-85
DOI https://doi.org/10.1016/j.molp.2018.10.009
Keywords cell expansion; cell-wall remodeling enzymes; endosperm; expansin; NAC transcription factors; seed germination
Public URL https://nottingham-repository.worktribe.com/output/1232252
Publisher URL https://www.sciencedirect.com/science/article/pii/S1674205218303356?via%3Dihub

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