Skip to main content

Research Repository

Advanced Search

Human cortical connectome reconstruction from diffusion weighted MRI: The effect of tractography algorithm

Bastiani, Matteo; Shah, Nadim Jon; Goebel, Rainer; Roebroeck, Alard


Matteo Bastiani

Nadim Jon Shah

Rainer Goebel

Alard Roebroeck


Reconstructing the macroscopic human cortical connectome by Diffusion Weighted Imaging (DWI) is a challenging research topic that has recently gained a lot of attention. In the present work, we investigate the effects of intra-voxel fiber direction modeling and tractography algorithm on derived structural network indices (e.g. density, small-worldness and global efficiency). The investigation is centered on three semi-independent distinctions within the large set of available diffusion models and tractography methods: i) single fiber direction versus multiple directions in the intra-voxel diffusion model, ii) deterministic versus probabilistic tractography and iii) local versus global measure-of-fit of the reconstructed fiber trajectories. The effect of algorithm and parameter choice has two components. First, there is the large effect of tractography algorithm and parameters on global network density, which is known to strongly affect graph indices. Second, and more importantly, there are remaining effects on graph indices which range in the tens of percent even when global density is controlled for. This is crucial for the sensitivity of any human structural network study and for the validity of study comparisons. We then investigate the effect of the choice of tractography algorithm on sensitivity and specificity of the resulting connections with a connectome dissection quality control (QC) approach. In this approach, evaluation of Tract Specific Density Coefficients (TSDCs) measures sensitivity while careful inspection of tractography path results assesses specificity. We use this to discuss interactions in the combined effects of these methods and implications for future studies.


Bastiani, M., Shah, N. J., Goebel, R., & Roebroeck, A. (2012). Human cortical connectome reconstruction from diffusion weighted MRI: The effect of tractography algorithm. NeuroImage, 62(3), 1732-1749.

Journal Article Type Article
Acceptance Date Jun 3, 2012
Online Publication Date Jun 12, 2012
Publication Date 2012-09
Deposit Date Oct 12, 2018
Publicly Available Date Oct 12, 2018
Journal NeuroImage
Print ISSN 1053-8119
Electronic ISSN 1095-9572
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 62
Issue 3
Pages 1732-1749
Public URL
Publisher URL


Downloadable Citations