If It Is Hard, It Is Worth Doing: Engineering Radical Enzymes from Anaerobes

Jäger, Christof M.; Croft, Anna K.

Abstract

With a pressing need for sustainable chemistries, radical enzymes from anaerobes offer a shortcut for many chemical transformations and deliver highly sought-after functionalizations such as late-stage C-H functionalization, C-C bond formation, and carbon-skeleton rearrangements, among others. The challenges in handling these oxygen-sensitive enzymes are reflected in their limited industrial exploitation, despite what they may deliver. With an influx of structures and mechanistic understanding, the scope for designed radical enzymes to deliver wanted processes becomes ever closer. Combined with new advances in computational methods and workflows for these complex systems, the outlook for an increased use of radical enzymes in future processes is exciting.

Citation

Jäger, C. M., & Croft, A. K. (2023). If It Is Hard, It Is Worth Doing: Engineering Radical Enzymes from Anaerobes. Biochemistry, 62(2), 241-252. https://doi.org/10.1021/acs.biochem.2c00376

• Journal Article Type: Article
• Acceptance Date: Sep 8, 2022
• Online Publication Date: Sep 19, 2022
• Publication Date: Jan 17, 2023
• Deposit Date: Sep 20, 2022
• Publicly Available Date: Sep 21, 2022
• Journal: Biochemistry
• Electronic ISSN: 1520-4995
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 62
• Issue: 2
• Pages: 241-252
• DOI: https://doi.org/10.1021/acs.biochem.2c00376
• Keywords: Biochemistry
• Public URL: https://nottingham-repository.worktribe.com/output/11464572
• Publisher URL: https://pubs.acs.org/doi/10.1021/acs.biochem.2c00376