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Self-sufficient flow-biocatalysis by coimmobilization of pyridoxal 5?-phosphate and ?-transaminases onto porous carriers


Ana I.

Martina L. Contente

Susana Velasco-Lozano

Francesca Paradisi



We expanded the application of self-sufficient heterogeneous biocatalysts containing coimmobilized w-transaminases and pyridoxal 5´-phosphate (PLP) to efficiently operate packed-bed reactors in continuous flow. Using a w-transaminase from Halomonas elongata co-immobilized with PLP onto porous methacrylate-based carriers coated with polyethyleneimine, we operated a packed-bed reactor continuously for up to 50 column volumes at 1.45 mL x min-1 in the enantioselective deamination of model amines (α-methylbenzyl amine), yielding > 90% conversion in all cycles without exogenous addition of cofactor. In this work, we expanded the concept of self-sufficient heterogeneous biocatalysts to other w-transaminases such as the ones from Chromobacterium violaceum and Pseudomonas fluorescens. We found that enzymes with lower affinities towards PLP present lower operational stabilities in flow, even when coimmobilizing PLP. Furthermore, w-transaminases co-immobilized with PLP were successfully implemented for the continuous synthesis of amines and the sustainable metrics were assessed. These results give some clues to exploit PLP-dependent w-transaminases under industrially relevant continuous operations in a more cost-effective and environmentally friendly process.


Benítez-Mateos, A. I., Contente, M. L., Velasco-Lozano, S., Paradisi, F., & López-Gallego, F. (2018). Self-sufficient flow-biocatalysis by coimmobilization of pyridoxal 5′-phosphate and ω-transaminases onto porous carriers. ACS Sustainable Chemistry and Engineering, 6(10), 13151-13159.

Journal Article Type Article
Acceptance Date Aug 26, 2018
Online Publication Date Aug 26, 2018
Publication Date Oct 1, 2018
Deposit Date Oct 1, 2018
Publicly Available Date Aug 27, 2019
Journal ACS Sustainable Chemistry & Engineering
Electronic ISSN 2168-0485
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 6
Issue 10
Pages 13151-13159
Public URL
Publisher URL
Additional Information This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Sustainable Chemistry and Engineering copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and Published work see


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