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Generation of amphidiploids from hybrids of wheat and related species from the genera Aegilops, Secale, Thinopyrum and Triticum as a source of genetic variation for wheat improvement

Nemeth, Csilla; Yang, Cai-yun; Kasprzak, Paul; Hubbart, Stella; Scholefield, Duncan; Mehra, Surbhi; Skipper, Emma; King, Ian; King, Julie

Authors

Csilla Nemeth

Cai-yun Yang

Paul Kasprzak

Stella Hubbart

Duncan Scholefield

Surbhi Mehra

Emma Skipper

Ian King



Abstract

We aim to improve diversity of domesticated wheat by transferring genetic variation for important target traits from related wild and cultivated grass species. The present study describes the development of F1 hybrids between wheat and related species from the genera Aegilops, Secale, Thinopyrum, and Triticum and production of new amphidiploids. Amphidiploid lines were produced from 20 different distant relatives. Both colchicine and caffeine were successfully used to double the chromosome numbers. The genomic constitution of the newly formed amphidiploids derived from seven distant relatives was determined using genomic in situ hybridization (GISH). Altogether, 42 different plants were analysed, 19 using multicolour GISH separating the chromosomes from the A, B, and D genomes of wheat, as well as the distant relative, and 23 using single colour GISH. Restructuring of the allopolyploid genome, both chromosome losses and aneuploidy, was detected in all the genomes contained by the amphidiploids. From the observed chromosome numbers there is an indication that in amphidiploids the B genome of wheat suffers chromosome losses less frequently than the other wheat genomes. Phenotyping to realize the full potential of the wheat – related grass germplasm is underway, linking the analyzed genotypes to agronomically important target traits.

Citation

Nemeth, C., Yang, C.-Y., Kasprzak, P., Hubbart, S., Scholefield, D., Mehra, S., Skipper, E., King, I., & King, J. (2015). Generation of amphidiploids from hybrids of wheat and related species from the genera Aegilops, Secale, Thinopyrum and Triticum as a source of genetic variation for wheat improvement. Genome, 58(2), 71-79. https://doi.org/10.1139/gen-2015-0002

Journal Article Type Article
Acceptance Date May 12, 2015
Online Publication Date May 14, 2015
Publication Date May 14, 2015
Deposit Date Sep 13, 2017
Print ISSN 0831-2796
Publisher NRC Research Press
Peer Reviewed Peer Reviewed
Volume 58
Issue 2
Pages 71-79
DOI https://doi.org/10.1139/gen-2015-0002
Public URL https://nottingham-repository.worktribe.com/output/1114408
Publisher URL http://www.nrcresearchpress.com/doi/10.1139/gen-2015-0002#.XFBh3Wngrb0