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FlhF(T368A) modulates motility in the bacteriophage carrier state of Campylobacter jejuni

Liang, Lu; Connerton, Ian F.

FlhF(T368A) modulates motility in the bacteriophage carrier state of Campylobacter jejuni Thumbnail


Authors

Lu Liang

IAN CONNERTON IAN.CONNERTON@NOTTINGHAM.AC.UK
Northern Foods Professor of Food Safety



Abstract

© 2018 The Authors. Molecular Microbiology Published by John Wiley & Sons Ltd. The carrier state is an alternative bacteriophage life cycle by which virulent bacteriophage can persist in association with host bacteria. Campylobacter jejuni carrier state strains exhibit growth phase dependent motility due to a truncated flagella phenotype. Genome sequencing identified a T368A substitution in the G3 domain of the SRP-like GTPase FlhF from C. jejuni PT14CP30A carrier state strains, which we hypothesized to be the cause of the complex motility phenotype. We have analyzed the role of this mutation in C. jejuni PT14 and demonstrated that flhF(T368A) leads to a large proportion of cells unable to synthesize flagella, while the remaining cells form a single flagellum at one pole leading to significantly reduced motility. The flhF(T368A) mutation causes a reduction in the phage adsorption constant, which leads to a decrease in infection efficiency. Down-regulation of σ28 and σ54 dependent flagellar genes were observed as responses to the flhF(T368A) mutation. FlhF(T368A) protein is impaired in GTPase activity and exhibits reduced stability. C. jejuni carrying flhF(T368A) are less sensitive to bacteriophage infection and formation of the carrier state. The acquisition of flhF(T368A) in carrier state strains acts to prevent super-infection and maintain association with the bacteriophage that provoked the interaction.

Citation

Liang, L., & Connerton, I. F. (2018). FlhF(T368A) modulates motility in the bacteriophage carrier state of Campylobacter jejuni. Molecular Microbiology, 110(4), 616-633. https://doi.org/10.1111/mmi.14120

Journal Article Type Article
Acceptance Date Sep 5, 2018
Online Publication Date Oct 23, 2018
Publication Date 2018-11
Deposit Date Sep 12, 2018
Publicly Available Date Mar 29, 2024
Journal Molecular Microbiology
Print ISSN 0950-382X
Electronic ISSN 1365-2958
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 110
Issue 4
Pages 616-633
DOI https://doi.org/10.1111/mmi.14120
Keywords Campylobacter, bacteriophage, carrier state life cycle, FlhF, motility
Public URL https://nottingham-repository.worktribe.com/output/1070776
Publisher URL https://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.14120

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