Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells

Sinjab, Faris; Awuah, Dennis; Gibson, Graham; Padgett, Miles; Ghaemmaghami, Amir M.; Notingher, Ioan

Abstract

We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy. Multiple laser foci, generated using a liquid-crystal spatial light modulator, are individually used for both optical trapping and excitation of spontaneous Raman spectroscopy from trapped objects. Raman scattering from each laser focus is spatially filtered using reflective apertures on a digital micro-mirror device, which can be reconfigured with flexible patterns at video rate. We discuss operation of the instrument, and performance and viability considerations for biological measurements. We then demonstrate the capability of the instrument for fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems.

Citation

Sinjab, F., Awuah, D., Gibson, G., Padgett, M., Ghaemmaghami, A. M., & Notingher, I. (2018). Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells. Optics Express, 26(19), 25211-25225. https://doi.org/10.1364/oe.26.025211

• Journal Article Type: Article
• Acceptance Date: Jul 30, 2018
• Online Publication Date: Sep 12, 2018
• Publication Date: Sep 17, 2018
• Deposit Date: Aug 15, 2018
• Publicly Available Date: Nov 1, 2019
• Journal: Optics Express
• Electronic ISSN: 1094-4087
• Publisher: Optical Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 26
• Issue: 19
• Article Number: 25211
• Pages: 25211-25225
• DOI: https://doi.org/10.1364/oe.26.025211
• Keywords: Atomic and Molecular Physics, and Optics
• Public URL: https://nottingham-repository.worktribe.com/output/1033929
• Publisher URL: https://www.osapublishing.org/oe/abstract.cfm?uri=oe-26-19-25211
• Additional Information: This article is maintained by: OSA - The Optical Society; Crossref DOI link to publisher maintained version: https://doi.org/10.1364/OE.26.025211; Article type: research-article; Similarity check: Screened by Similarity Check; Peer reviewed: Yes; Review process: Single blind; Received: 13 June 2018; Accepted: 20 July 2018; Published: 12 September 2018; Copyright: Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.