High glucose mediates prooxidant and antioxidant enzyme activities in coronary endothelial cells

Bayraktutan, Ulvi; Weidig, Pamela; McMaster, Dorothy; Ulker, Sibel

Abstract

Objective: Excess levels of free radicals such as nitric oxide (NO) and superoxide anion (O2-)are associated with the pathogenesis of endothelial cell dysfunction in diabetes mellitus. This study was designed to investigate the underlying causes of oxidative stress in coronary microvascular endothelial cells (CMEC) exposed to hyperglycaemia.

Methods: CMEC were cultured under normal (5.5 mmol/L) or high glucose (22 mmol/L)concentrations for 7 days. The activity and expression (protein level) of eNOS, iNOS, NAD(P)H oxidase and antioxidant enzymes, namely, superoxide dismutase (SOD), catalase and glutahione peroxidase (GPx) were investigated by specific activity assays and Western analyses,respectively while the effects of hyperglycaemia on nitrite and O2 - generation were investigated
by Griess reaction and cytochrome C reduction assay, respectively.

Results: Hyperglycaemia did not alter eNOS or iNOS protein expressions and overall nitrite generation, an index of NO production. However, it significantly reduced the levels of intracellular antioxidant glutathione by 50% (p

Citation

Bayraktutan, U., Weidig, P., McMaster, D., & Ulker, S. (2004). High glucose mediates prooxidant and antioxidant enzyme activities in coronary endothelial cells

• Journal Article Type: Article
• Publication Date: Jan 1, 2004
• Deposit Date: Apr 17, 2007
• Publicly Available Date: Oct 9, 2007
• Journal: Diabetes Obesity and metabolism
• Peer Reviewed: Not Peer Reviewed
• Volume: 6
• Public URL: https://nottingham-repository.worktribe.com/output/1021213