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Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes

Stepek, Gillian; Curtis, R.H.C.; Kerry, B.R.; Shewry, P.R.; Clark, S.J.; Lowe, Ann; Duce, I.R.; Buttle, David J.; Behnke, J.M.

Authors

Gillian Stepek

R.H.C. Curtis

B.R. Kerry

P.R. Shewry

S.J. Clark

Ann Lowe

I.R. Duce

David J. Buttle

J.M. Behnke



Abstract

Cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. In this paper, we describe the in vitro effects of these plant extracts against 2 sedentary plant parasitic nematodes of the genera Meloidogyne and Globodera. All the plant extracts examined caused digestion of the cuticle and decreased the activity of the tested nematodes. The specific inhibitor of cysteine proteinases, E-64, blocked this activity completely, indicating that it was essentially mediated by cysteine proteinases. In vitro, plant cysteine proteinases are active against second-stage juveniles of M. incognita and M. javanica, and some cysteine proteinases also affect the second-stage juveniles of Globodera rostochiensis. It is not known yet whether these plant extracts will interfere with, or prevent invasion of, host plants.

Citation

Stepek, G., Curtis, R., Kerry, B., Shewry, P., Clark, S., Lowe, A., …Behnke, J. (2007). Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes. Parasitology, 134(12), doi:10.1017/S0031182007003289

Journal Article Type Article
Publication Date Jan 1, 2007
Deposit Date Jul 27, 2015
Publicly Available Date Jul 27, 2015
Journal Parasitology
Print ISSN 0031-1820
Electronic ISSN 0031-1820
Publisher Cambridge University Press (CUP)
Peer Reviewed Peer Reviewed
Volume 134
Issue 12
DOI https://doi.org/10.1017/S0031182007003289
Keywords plant parasitic nematodes, Meloidogyne spp., Globodera rostochiensis, plant cysteine proteinases, in vitro, nematicide
Public URL http://eprints.nottingham.ac.uk/id/eprint/29417
Publisher URL http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1367720&fileId=S0031182007003289
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf
Additional Information Copyright Cambridge University Press.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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