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Botanical extracts from rosehip (rosa canina), willow bark (salix alba), and nettle leaf (urtica dioica) suppress IL-1β-induced NF-κB activation in canine articular chondrocytes

Shakibaei, Mehdi; Allaway, David; Nebrich, Simone; Mobasheri, Ali

Authors

Mehdi Shakibaei

David Allaway

Simone Nebrich

Ali Mobasheri ali.mobasheri@nottingham.ac.uk



Abstract

The aim of this study was to characterize the anti-inflammatory mode of action of botanical extracts from rosehip (Rosa canina), willow bark (Salix alba), and nettle leaf (Urtica dioica) in an in vitro model of primary canine articular chondrocytes. Methods. The biological effects of the botanical extracts were studied in chondrocytes treated with IL-1β for up to 72 h. Expression of collagen type II, cartilage-specific proteoglycan (CSPG), β1-integrin, SOX-9, COX-2, and MMP-9 and MMP-13 was examined by western blotting. Results. The botanical extracts suppressed IL-1β-induced NF-κB activation by inhibition of IκBα phosphorylation, IκBα degradation, p65 phosphorylation, and p65 nuclear translocation. These events correlated with downregulation of NF-κB targets including COX-2 and MMPs. The extracts also reversed the IL-1β-induced downregulation of collagen type II, CSPG, β1-integrin, and cartilage-specific transcription factor SOX-9 protein expression. In high-density cultures botanical extracts stimulated new cartilage formation even in the presence of IL-1β. Conclusions. Botanical extracts exerted anti-inflammatory and anabolic effects on chondrocytes. The observed reduction of IL-1β-induced NF-κB activation suggests that further studies are warranted to demonstrate the effectiveness of plant extracts in the treatment of OA and other conditions in which NF-κB plays pathophysiological roles.

Journal Article Type Article
Publication Date Jan 1, 2012
Journal Evidence-Based Complementary and Alternative Medicine
Print ISSN 1741-427X
Electronic ISSN 1741-427X
Publisher Hindawi Publishing Corporation
Peer Reviewed Peer Reviewed
Volume 2012
Article Number 16
APA6 Citation Shakibaei, M., Allaway, D., Nebrich, S., & Mobasheri, A. (2012). Botanical extracts from rosehip (rosa canina), willow bark (salix alba), and nettle leaf (urtica dioica) suppress IL-1β-induced NF-κB activation in canine articular chondrocytes. Evidence-Based Complementary and Alternative Medicine, 2012, doi:10.1155/2012/509383
DOI https://doi.org/10.1155/2012/509383
Publisher URL http://www.hindawi.com/journals/ecam/2012/509383/
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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