Mehdi Shakibaei
Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1?-Induced NF-?B Activation in Canine Articular Chondrocytes
Shakibaei, Mehdi; Allaway, David; Nebrich, Simone; Mobasheri, Ali
Authors
David Allaway
Simone Nebrich
Ali Mobasheri
Abstract
The aim of this study was to characterize the anti-inflammatory mode of action of botanical extracts from rosehip (Rosa canina), willow bark (Salix alba), and nettle leaf (Urtica dioica) in an in vitro model of primary canine articular chondrocytes. Methods. The biological effects of the botanical extracts were studied in chondrocytes treated with IL-1? for up to 72?h. Expression of collagen type II, cartilage-specific proteoglycan (CSPG), ?1-integrin, SOX-9, COX-2, and MMP-9 and MMP-13 was examined by western blotting. Results. The botanical extracts suppressed IL-1?-induced NF-?B activation by inhibition of I?B? phosphorylation, I?B? degradation, p65 phosphorylation, and p65 nuclear translocation. These events correlated with downregulation of NF-?B targets including COX-2 and MMPs. The extracts also reversed the IL-1?-induced downregulation of collagen type II, CSPG, ?1-integrin, and cartilage-specific transcription factor SOX-9 protein expression. In high-density cultures botanical extracts stimulated new cartilage formation even in the presence of IL-1?. Conclusions. Botanical extracts exerted anti-inflammatory and anabolic effects on chondrocytes. The observed reduction of IL-1?-induced NF-?B activation suggests that further studies are warranted to demonstrate the effectiveness of plant extracts in the treatment of OA and other conditions in which NF-?B plays pathophysiological roles.
Citation
Shakibaei, M., Allaway, D., Nebrich, S., & Mobasheri, A. (2012). Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1?-Induced NF-?B Activation in Canine Articular Chondrocytes. Evidence-Based Complementary and Alternative Medicine, 2012, Article 509383. https://doi.org/10.1155/2012/509383
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 10, 2011 |
Online Publication Date | Mar 14, 2012 |
Publication Date | 2012 |
Deposit Date | Apr 11, 2014 |
Publicly Available Date | Apr 11, 2014 |
Journal | Evidence-Based Complementary and Alternative Medicine |
Print ISSN | 1741-427X |
Electronic ISSN | 1741-4288 |
Publisher | Hindawi Publishing Corporation |
Peer Reviewed | Peer Reviewed |
Volume | 2012 |
Article Number | 509383 |
DOI | https://doi.org/10.1155/2012/509383 |
Keywords | Complementary and alternative medicine |
Public URL | https://nottingham-repository.worktribe.com/output/1009275 |
Publisher URL | http://www.hindawi.com/journals/ecam/2012/509383/ |
Files
509383.pdf
(2 Mb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: digital-library-support@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search