Development of a new SonovueTM contrast-enhanced ultrasound approach reveals temporal and age-related features of muscle microvascular responses to feeding

Mitchell, William Kyle; Phillips, Bethan E.; Williams, John P.; Rankin, Debbie; Smith, Kenneth; Lund, Jonathan N.; Atherton, Philip J.

Abstract

Compromised limb blood flow in aging may contribute to the development of sarcopenia, frailty, and the metabolic syndrome. We developed a novel contrast-enhanced ultrasound technique using Sonovue™ to characterize muscle microvasculature responses to an oral feeding stimulus (15 g essential amino acids) in young (~20 years) and older (~70 years) men. Intensity-time replenishment curves were made via an ultrasound probe “fixed” over the quadriceps, with intermittent high mechanical index destruction of microbubbles within muscle vasculature. This permitted real-time measures of microvascular blood volume (MBV), microvascular flow velocity (MFV) and their product, microvascular blood flow (MBF). Leg blood flow (LBF) was measured by Doppler and insulin by enzyme-linked immunosorbent assay. Steady-state contrast concentrations needed for comparison between different physiological states were achieved

Citation

Mitchell, W. K., Phillips, B. E., Williams, J. P., Rankin, D., Smith, K., Lund, J. N., & Atherton, P. J. (2013). Development of a new SonovueTM contrast-enhanced ultrasound approach reveals temporal and age-related features of muscle microvascular responses to feeding. Physiological Reports, 1(5), Article e00119. https://doi.org/10.1002/phy2.119

• Journal Article Type: Article
• Publication Date: Oct 1, 2013
• Deposit Date: Apr 22, 2014
• Publicly Available Date: Apr 22, 2014
• Journal: Physiological Reports
• Electronic ISSN: 2051-817X
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 1
• Issue: 5
• Article Number: e00119
• DOI: https://doi.org/10.1002/phy2.119
• Public URL: https://nottingham-repository.worktribe.com/output/1001131
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/phy2.119/abstract