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Development of a porous poly(DL-lactic acid-co-glycolic acid)-based scaffold for mastoid air-cell regeneration

Gould, Toby W.A.; Birchall, John P.; Mallick, Ali S.; Alliston, Tamara; Lustig, Lawrence R.; Shakesheff, Kevin M.; Rahman, Cheryl V.

Authors

Toby W.A. Gould

John P. Birchall

Ali S. Mallick

Tamara Alliston

Lawrence R. Lustig

Kevin M. Shakesheff

Cheryl V. Rahman



Abstract

Objectives/Hypothesis: To develop a porous, biodegradable scaffold for mastoid air-cell regeneration.
Study Design: In vitro development of a temperature-sensitive poly(DL-lactic acid-co-glycolic acid)/poly(ethylene glycol) (PLGA/PEG) scaffold tailored for this application.
Methods: Human mastoid bone microstructure and porosity were investigated using micro-computed tomography.
PLGA/PEG-alginate scaffolds were developed, and scaffold porosity was assessed. Human bone marrow mesenchymal stem
cells (hBM-MSCs) were cultured on the scaffolds in vitro. Scaffolds were loaded with ciprofloxacin, and release of ciprofloxacin over time in vitro was assessed.
Results: Porosity of human mastoid bone was measured at 83% with an average pore size of 1.3 mm. PLGA/PEG-alginate
scaffold porosity ranged from 43% to 78% depending on the alginate bead content. The hBM-MSCs proliferate on the
scaffolds in vitro, and release of ciprofloxacin from the scaffolds was demonstrated over 7 to 10 weeks.
Conclusions: The PLGA/PEG-alginate scaffolds developed in this study demonstrate similar structural features to human mastoid bone, support cell growth, and display sustained antibiotic release. These scaffolds may be of potential clinical use in mastoid air-cell regeneration. Further in vivo studies to assess the suitability of PLGA/PEG-alginate scaffolds for this application are required.
Key Words: Scaffold, poly(DL-lactic acid-co-glycolic acid), alginate, mastoid, ciprofloxacin.
Level of Evidence: N/A.

Journal Article Type Article
Publication Date Dec 1, 2013
Journal The Laryngoscope.
Electronic ISSN 0023-852X
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 123
Issue 12
APA6 Citation Gould, T. W., Birchall, J. P., Mallick, A. S., Alliston, T., Lustig, L. R., Shakesheff, K. M., & Rahman, C. V. (2013). Development of a porous poly(DL-lactic acid-co-glycolic acid)-based scaffold for mastoid air-cell regeneration. Laryngoscope, 123(12), doi:10.1002/lary.24173
DOI https://doi.org/10.1002/lary.24173
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/lary.24173/abstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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