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Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of ?-arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors (2012)
Journal Article
Kilpatrick, L. E., Briddon, S. J., & Holliday, N. D. (2012). Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of ?-arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1823(6),

Fluorescence correlation spectroscopy (FCS) and photon counting histogram (PCH) analysis are powerful ways to study mobility and stoichiometry of G protein coupled receptor complexes, within microdomains of single living cells. However, relating thes... Read More about Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of ?-arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors.

Fluoxetine counteracts the cognitive and cellular effects of 5-Fluorouracil in the rat hippocampus by a mechanism of prevention rather than recovery (2012)
Journal Article
Lyons, L., ELBeltagy, M., Bennett, G., & Wigmore, P. (2012). Fluoxetine counteracts the cognitive and cellular effects of 5-Fluorouracil in the rat hippocampus by a mechanism of prevention rather than recovery. PLoS ONE, 7(1), Article e30010. https://doi.org/10.1371/journal.pone.0030010

5-Fluorouracil (5-FU) is a cytostatic drug associated with chemotherapy-induced cognitive impairments that many cancer patients experience after treatment. Previous work in rodents has shown that 5-FU reduces hippocampal cell proliferation, a possi... Read More about Fluoxetine counteracts the cognitive and cellular effects of 5-Fluorouracil in the rat hippocampus by a mechanism of prevention rather than recovery.

Predicting in vivo cardiovascular properties of ?-blockers from cellular assays: a quantative comparison of cellular and cardiovascular pharmacological responses (2011)
Journal Article
Baker, J. G., Kemp, P., March, J., Fretwell, L., Hill, S. J., & Gardiner, S. M. (2011). Predicting in vivo cardiovascular properties of ?-blockers from cellular assays: a quantative comparison of cellular and cardiovascular pharmacological responses. FASEB Journal, 25(12), https://doi.org/10.1096/fj.11-192435

?-Adrenoceptor antagonists differ in their degree of partial agonism. In vitro assays have provided information on ligand affinity, selectivity, and intrinsic efficacy. However, the extent to which these properties are manifest in vivo is less clear.... Read More about Predicting in vivo cardiovascular properties of ?-blockers from cellular assays: a quantative comparison of cellular and cardiovascular pharmacological responses.

Dimerization of ABCG2 analysed by bimolecular fluorescence complementation (2011)
Journal Article
Haider, A. J., Briggs, D., Self, T. J., Chilvers, H. L., Holliday, N. D., & Kerr, I. D. (2011). Dimerization of ABCG2 analysed by bimolecular fluorescence complementation. PLoS ONE, 6(10), Article e25818. https://doi.org/10.1371/journal.pone.0025818

ABCG2 is one of three human ATP binding cassette transporters that are functionally capable of exporting a diverse range of substrates from cells. The physiological consequence of ABCG2 multidrug transport activity in leukaemia, and some solid tumour... Read More about Dimerization of ABCG2 analysed by bimolecular fluorescence complementation.

Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart (2011)
Journal Article
Rutland, C. S., Polo-Parada, L., Ehler, E., Alibhai, A., Thorpe, A., Suren, S., …Loughna, S. (2011). Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart. Development, 138(18), https://doi.org/10.1242/dev.059063

The expression and function of embryonic myosin heavy chain (eMYH) has not been investigated within the early developing heart. This is despite the knowledge that other structural proteins, such as alpha and beta myosin heavy chains and cardiac alpha... Read More about Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart.

Functional network construction in Arabidopsis using rule-based machine learning on large-scale data sets (2011)
Journal Article
Bassel, G. W., Glaab, E., Marquez, J., Holdsworth, M. J., & Barcardit, J. (2011). Functional network construction in Arabidopsis using rule-based machine learning on large-scale data sets. Plant Cell, 23(9), https://doi.org/10.1105/tpc.111.088153

The meta-analysis of large-scale postgenomics data sets within public databases promises to provide important novel biological knowledge. Statistical approaches including correlation analyses in coexpression studies of gene expression have emerged as... Read More about Functional network construction in Arabidopsis using rule-based machine learning on large-scale data sets.

SUMO conjugation of STAT1 protects cells from hyperresponsiveness to IFN? (2011)
Journal Article
Begitt, A., Droescher, M., Knobeloch, K., & Vinkemeier, U. (2011). SUMO conjugation of STAT1 protects cells from hyperresponsiveness to IFN?. Blood, 118(4), https://doi.org/10.1182/blood-2011-04-347930

The biologic effects of IFNγ are mediated by the transcription factor STAT1. The activity of STAT1 is inhibited by small ubiquitin-like modifier (SUMO) conjugation. This occurs both directly through decreasing STAT1 tyrosine phosphorylation and indir... Read More about SUMO conjugation of STAT1 protects cells from hyperresponsiveness to IFN?.

STAT1 signaling is not regulated by a phosphorylation-acetylation switch (2011)
Journal Article
Antunes, F., Marg, A., & Vinkemeier, U. (2011). STAT1 signaling is not regulated by a phosphorylation-acetylation switch. Molecular and Cellular Biology, 31(14), https://doi.org/10.1128/MCB.05300-11

The treatment of cells with histone deacetylase inhibitors (HDACi) was reported to reveal the acetylation of STAT1 at lysine 410 and lysine 413 (O. H. Kra¨mer et al., Genes Dev. 20:473–485, 2006). STAT1 acetylation was proposed to regulate apoptosi... Read More about STAT1 signaling is not regulated by a phosphorylation-acetylation switch.

H-NS mediates the dissociation of a refractory protein–DNA complex during Tn10/IS10 transposition (2011)
Journal Article
Liu, D., Haniford, D. B., & Chalmers, R. (2011). H-NS mediates the dissociation of a refractory protein–DNA complex during Tn10/IS10 transposition. Nucleic Acids Research, 39(15), https://doi.org/10.1093/nar/gkr309

Tn10/IS10 transposition takes place in the context of a protein–DNA complex called a transpososome. During the reaction, the transpososome undergoes several conformational changes. The host proteins IHF and H-NS, which also are global regulators of g... Read More about H-NS mediates the dissociation of a refractory protein–DNA complex during Tn10/IS10 transposition.