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Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing (2023)
Journal Article
Lever, R., Simmons, E., Gamble-Milner, R., Buckley, R., Harrison, C., Parkes, A., …Allers, T. (2023). Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing. Nucleic Acids Research, https://doi.org/10.1093/nar/gkad572

Hel308 helicases promote genome stability in archaea and are conserved in metazoans, where they are known as HELQ. Their helicase mechanism is well characterised, but it is unclear how they specifically contribute to genome stability in archaea. We s... Read More about Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing.

Halofrax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems (2019)
Journal Article
Haque, R., Paradisi, F., & Allers, T. (2019). Halofrax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems. Applied Microbiology and Biotechnology, 103(9), 3807-3817. https://doi.org/10.1007/s00253-019-09725-y

Enzyme-mediated synthesis of pharmaceutical compounds is a ‘green’ alternative to traditional synthetic chemistry, and microbial engineering opens up the possibility of using whole cells as mini-factories. Whole-cell biocatalysis reduces cost by elim... Read More about Halofrax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems.

Haloferax volcanii for biotechnology applications: challenges, current state and perspectives (2019)
Journal Article
Haque, R. U., Paradisi, F., & Allers, T. (2019). Haloferax volcanii for biotechnology applications: challenges, current state and perspectives. Applied Microbiology and Biotechnology, 1-12. https://doi.org/10.1007/s00253-019-10314-2

© 2019, The Author(s). Haloferax volcanii is an obligate halophilic archaeon with its origin in the Dead Sea. Simple laboratory culture conditions and a wide range of genetic tools have made it a model organism for studying haloarchaeal cell biology.... Read More about Haloferax volcanii for biotechnology applications: challenges, current state and perspectives.

DNA repair in the archaea: an emerging picture (2018)
Journal Article
White, M. F., & Allers, T. (2018). DNA repair in the archaea: an emerging picture. FEMS Microbiology Reviews, 42(4), 514-526. https://doi.org/10.1093/femsre/fuy020

There has long been a fascination in the DNA Repair pathways of archaea, for two main reasons. Firstly, many archaea inhabit extreme environments where the rate of physical damage to DNA is accelerated. These archaea might reasonably be expected to h... Read More about DNA repair in the archaea: an emerging picture.

Evolution of genome architecture in archaea: spontaneous generation of a new chromosome in Haloferax volcanii (2018)
Journal Article
Ausiannikava, D., Mitchell, L., Marriott, H., Smith, V., Hawkins, M., Makarova, K. S., …Allers, T. (2018). Evolution of genome architecture in archaea: spontaneous generation of a new chromosome in Haloferax volcanii. Molecular Biology and Evolution, 35(8), 1855-1868. https://doi.org/10.1093/molbev/msy075

The common ancestry of archaea and eukaryotes is evident in their genome architecture. All eukaryotic and several archaeal genomes consist of multiple chromosomes, each replicated from multiple origins. Three scenarios have been proposed for the evol... Read More about Evolution of genome architecture in archaea: spontaneous generation of a new chromosome in Haloferax volcanii.

RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator (2017)
Journal Article
Wardell, K., Haldenby, S., Jones, N., Liddell, S., Ngo, G. H., & Allers, T. (2017). RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator. DNA Repair, 55, https://doi.org/10.1016/j.dnarep.2017.04.005

Homologous recombination plays a central role in the repair of double-strand DNA breaks, the restart of stalled replication forks and the generation of genetic diversity. Regulation of recombination is essential since defects can lead to genome insta... Read More about RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator.

High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon (2017)
Journal Article
Stachler, A., Turgeman-Grott, I., Shtifman-Segal, E., Allers, T., Marchfelder, A., & Gophna, U. (2017). High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon. Nucleic Acids Research, 45(9), 5208-5216. https://doi.org/10.1093/nar/gkx150

CRISPR-Cas systems allow bacteria and archaea to acquire sequence-specific immunity against selfish genetic elements such as viruses and plasmids, by specific degradation of invader DNA or RNA. However, this involves the risk of autoimmunity if immun... Read More about High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon.

Diversity of DNA replication in the archaea (2017)
Journal Article
Ausiannikava, D., & Allers, T. (2017). Diversity of DNA replication in the archaea. Genes, 8(2), https://doi.org/10.3390/genes8020056

DNA replication is arguably the most fundamental biological process. On account of their shared evolutionary ancestry, the replication machinery found in archaea is similar to that found in eukaryotes. DNA replication is initiated at origins and is h... Read More about Diversity of DNA replication in the archaea.

Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor (2015)
Journal Article
Strillinger, E., Grötzinger, S. W., Allers, T., Eppinger, J., & Weuster-Botz, D. (2016). Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor. Applied Microbiology and Biotechnology, 100(3), 1183-1195. https://doi.org/10.1007/s00253-015-7007-1

The success of biotechnological processes is based on the availability of efficient and highly specific biocatalysts, which can satisfy industrial demands. Extreme and remote environments like the deep brine pools of the Red Sea represent highly inte... Read More about Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor.

Genetic and biochemical identification of a novel single-stranded DNA-binding complex in Haloferax volcanii (2012)
Journal Article
Stroud, A., Liddell, S., & Allers, T. (2012). Genetic and biochemical identification of a novel single-stranded DNA-binding complex in Haloferax volcanii. Frontiers in Microbiology, 3, Article 224. https://doi.org/10.3389/fmicb.2012.00224

Single-stranded DNA (ssDNA)-binding proteins play an essential role in DNA replication and repair. They use oligonucleotide/oligosaccharide-binding (OB)-folds, a five-stranded ?-sheet coiled into a closed barrel, to bind to ssDNA thereby protecting a... Read More about Genetic and biochemical identification of a novel single-stranded DNA-binding complex in Haloferax volcanii.