Outputs (9)

Targeted DNA transposition in vitro using a dCas9-transposase fusion protein (2019)
Journal Article
Bhatt, S., & Chalmers, R. (2019). Targeted DNA transposition in vitro using a dCas9-transposase fusion protein. Nucleic Acids Research, 47(15), 8126–8135. https://doi.org/10.1093/nar/gkz552

Homology-directed genome engineering is limited by transgene size. Although DNA transposons are more efficient with large transgenes, random integrations are potentially mutagenic. Here we present an in vitro mechanistic study that demonstrates effic... Read More about Targeted DNA transposition in vitro using a dCas9-transposase fusion protein.

The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair (2019)
Journal Article
Tellier, M., & Chalmers, R. (2019). The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair. DNA Repair, 80, 26-35. https://doi.org/10.1016/j.dnarep.2019.06.006

SETMAR is a fusion between a SET-domain methyltransferase gene and a mariner-family transposase gene, which is specific to anthropoid primates. However, the ancestral SET gene is present in all other mammals and birds. SETMAR is reported to be involv... Read More about The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair.

Human SETMAR is a DNA sequence-specific histone-methylase with a broad effect on the transcriptome (2018)
Journal Article
Tellier, M., & Chalmers, R. (2019). Human SETMAR is a DNA sequence-specific histone-methylase with a broad effect on the transcriptome. Nucleic Acids Research, 47(1), 122–133. https://doi.org/10.1093/nar/gky937

Transposons impart dynamism to the genomes they inhabit and their movements frequently rewire the control of nearby genes. Occasionally, their proteins are domesticated when they evolve a new function. SETMAR is a protein methylase with a sequence-sp... Read More about Human SETMAR is a DNA sequence-specific histone-methylase with a broad effect on the transcriptome.

Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes (2018)
Journal Article
Blundell-Hunter, G., Tellier, M., & Chalmers, R. (2018). Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes. Nucleic Acids Research, 46(18), 9637-9646. https://doi.org/10.1093/nar/gky794

Cut-and-paste transposons are important tools for mutagenesis, gene-delivery and DNA sequencing applications. At the molecular level, the most thoroughly understood are Tn5 and Tn10 in bacteria, and mariner and hAT elements in eukaryotes. All bacteri... Read More about Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes.

The invertebrate lysozyme effector ILYS-3 is systemically activated in response to danger signals and confers antimicrobial protection in C. elegans (2016)
Journal Article
Gravato-Nobre, M. J., Vaz, F., Filipe, S., Chalmers, R., & Hodgkin, J. (2016). The invertebrate lysozyme effector ILYS-3 is systemically activated in response to danger signals and confers antimicrobial protection in C. elegans. PLoS Pathogens, 12(8), Article e1005826. https://doi.org/10.1371/journal.ppat.1005826

Little is known about the relative contributions and importance of antibacterial effectors in the nematode C. elegans, despite extensive work on the innate immune responses in this organism. We report an investigation of the expression, function and... Read More about The invertebrate lysozyme effector ILYS-3 is systemically activated in response to danger signals and confers antimicrobial protection in C. elegans.

One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition (2014)
Journal Article
Bouuaert, C. C., Chalmers, R., & Tellier, M. (2014). One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition. Mobile Genetic Elements, 4(2), https://doi.org/10.4161/mge.28807

The development of transposon-based genome manipulation tools can benefit greatly from understanding transposons’ inherent regulatory mechanisms. The Tc1-mariner transposons, which are being widely used in biotechnological applications, are subject t... Read More about One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition.

Crosstalk between transposase subunits during cleavage of the mariner transposon (2014)
Journal Article
Claeys Bouuaert, C., Walker, N., Liu, D., & Chalmers, R. (2014). Crosstalk between transposase subunits during cleavage of the mariner transposon. Nucleic Acids Research, 42(9), https://doi.org/10.1093/nar/gku172

Mariner transposition is a complex reaction that involves three recombination sites and six strand breaking and joining reactions. This requires precise spatial and temporal coordination between the different components to ensure a productive outcome... Read More about Crosstalk between transposase subunits during cleavage of the mariner transposon.

Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis (2013)
Journal Article
Liu, D., & Chalmers, R. (2014). Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis. Nucleic Acids Research, 42(4), https://doi.org/10.1093/nar/gkt1218

New applications for transposons in vertebrate genetics have spurred efforts to develop hyperactive variants. Typically, a genetic screen is used to identify several hyperactive point mutations, which are then incorporated in a single transposase gen... Read More about Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis.

Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome (2013)
Journal Article
Xu, Z., Thomas, L., Davies, B., Chalmers, R., Smith, M., & Brown, W. (2013). Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome. BMC Biotechnology, 13, Article 87. https://doi.org/10.1186/1472-6750-13-87

Background: Phage-encoded serine integrases, such as ?C31 integrase, are widely used for genome engineering. Fifteen such integrases have been described but their utility for genome engineering has not been compared in uniform assays. Results: We h... Read More about Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome.