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Outputs (73)

Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum (2023)
Journal Article
Poulalier-Delavelle, M., Baker, J. P., Millard, J., Winzer, K., & Minton, N. P. (2023). Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum. Frontiers in Bioengineering and Biotechnology, 11, Article 1213236. https://doi.org/10.3389/fbioe.2023.1213236

Acetogenic bacteria can play a major role in achieving Net Zero through their ability to convert CO2 into industrially relevant chemicals and fuels. Full exploitation of this potential will be reliant on effective metabolic engineering tools, such as... Read More about Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum.

Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations (2023)
Journal Article
Seys, F. M., Humphreys, C. M., Tomi-Andrino, C., Li, Q., Millat, T., Yang, S., & Minton, N. P. (2023). Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations. Frontiers in Bioengineering and Biotechnology, 11, Article 1211197. https://doi.org/10.3389/fbioe.2023.1211197

Base editors are recent multiplex gene editing tools derived from the Cas9 nuclease of Streptomyces pyogenes. They can target and modify a single nucleotide in the genome without inducing double-strand breaks (DSB) of the DNA helix. As such, they hol... Read More about Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations.

Metabolic engineering of Cupriavidus necator H16 for heterotrophic and autotrophic production of 3-hydroxypropionic acid (2022)
Journal Article
Salinas, A., McGregor, C., Irorere, V., Arenas-López, C., Bommareddy, R. R., Winzer, K., …Kovács, K. (2022). Metabolic engineering of Cupriavidus necator H16 for heterotrophic and autotrophic production of 3-hydroxypropionic acid. Metabolic Engineering, 74, 178-190. https://doi.org/10.1016/j.ymben.2022.10.014

3-Hydroxypropionate (3-HP) is a versatile compound for chemical synthesis and a potential building block for biodegradable polymers. Cupriavidus necator H16, a facultative chemolithoautotroph, is an attractive production chassis and has been extensiv... Read More about Metabolic engineering of Cupriavidus necator H16 for heterotrophic and autotrophic production of 3-hydroxypropionic acid.

A genome-scale metabolic model of Cupriavidus necator H16 integrated with TraDIS and transcriptomic data reveals metabolic insights for biotechnological applications (2022)
Journal Article
Pearcy, N., Garavaglia, M., Millat, T., Gilbert, J. P., Song, Y., Hartman, H., …Minton, N. P. (2022). A genome-scale metabolic model of Cupriavidus necator H16 integrated with TraDIS and transcriptomic data reveals metabolic insights for biotechnological applications. PLoS Computational Biology, 18(5), Article e1010106. https://doi.org/10.1371/journal.pcbi.1010106

Exploiting biological processes to recycle renewable carbon into high value platform chemicals provides a sustainable and greener alternative to current reliance on petrochemicals. In this regard Cupriavidus necator H16 represents a particularly prom... Read More about A genome-scale metabolic model of Cupriavidus necator H16 integrated with TraDIS and transcriptomic data reveals metabolic insights for biotechnological applications.

Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum (2022)
Journal Article
Woods, C., Humphreys, C. M., Tomi-Andrino, C., Henstra, A. M., Köpke, M., Simpson, S. D., …Minton, N. P. (2022). Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum. Applied and Environmental Microbiology, 88(7), https://doi.org/10.1128/aem.02479-21

The majority of the genes present in bacterial genomes remain poorly characterized, with up to one-third of those that are protein encoding having no definitive function. Transposon insertion sequencing represents a high-throughput technique that can... Read More about Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum.

Establishing Mixotrophic Growth of Cupriavidus necator H16 on CO2 and Volatile Fatty Acids (2022)
Journal Article
Jawed, K., Irorere, V. U., Bommareddy, R. R., Minton, N. P., & Kovács, K. (2022). Establishing Mixotrophic Growth of Cupriavidus necator H16 on CO2 and Volatile Fatty Acids. Fermentation, 8(3), Article 125. https://doi.org/10.3390/fermentation8030125

The facultative chemolithoautotroph Cupriavidus necator H16 is able to grow aerobically either with organic substrates or H2 and CO2 s and it can accumulate large amounts of (up to 90%) poly (3‐hydroxybutyrate), a polyhydroxyalkanoate (PHA) biopolyme... Read More about Establishing Mixotrophic Growth of Cupriavidus necator H16 on CO2 and Volatile Fatty Acids.

Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production (2022)
Journal Article
Hanko, E. K., Sherlock, G., Minton, N. P., & Malys, N. (2022). Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production. Metabolic Engineering, 72, 24-34. https://doi.org/10.1016/j.ymben.2022.02.003

Cupriavidus necator H16 is one of the most researched carbon dioxide (CO2)-fixing bacteria. It can store carbon in form of the polymer polyhydroxybutyrate and generate energy by aerobic hydrogen oxidation under lithoautotrophic conditions, making C.... Read More about Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production.

A Novel Bacteriophage with Broad Host Range against Clostridioides difficile Ribotype 078 Supports SlpA as the Likely Phage Receptor (2022)
Journal Article
Whittle, M. J., Bilverstone, T. W., Van Esveld, R. J., Lücke, A. C., Lister, M. M., Kuehne, S. A., & Minton, N. P. (2022). A Novel Bacteriophage with Broad Host Range against Clostridioides difficile Ribotype 078 Supports SlpA as the Likely Phage Receptor. Microbiology Spectrum, 10(1), Article e02295-21. https://doi.org/10.1128/spectrum.02295-21

Bacteriophages represent a promising option for the treatment of Clostridioides difficile (formerly Clostridium difficile) infection (CDI), which at present relies on conventional antibiotic therapy. The specificity of bacteriophages should prevent d... Read More about A Novel Bacteriophage with Broad Host Range against Clostridioides difficile Ribotype 078 Supports SlpA as the Likely Phage Receptor.

Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum (2022)
Journal Article
Piatek, P., Humphreys, C., Raut, M., Wright, P. C., Simpson, S., Köpke, M., …Winzer, K. (2022). Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum. Scientific Reports, 12(1), Article 411. https://doi.org/10.1038/s41598-021-03999-x

Acetogenic bacteria are capable of fermenting CO2 and carbon monoxide containing waste-gases into a range of platform chemicals and fuels. Despite major advances in genetic engineering and improving these biocatalysts, several important physiological... Read More about Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum.

Biosynthesis of Poly(3HB- co-3HP) with Variable Monomer Composition in Recombinant Cupriavidus necator H16 (2021)
Journal Article
McGregor, C., Minton, N. P., & Kovács, K. (2021). Biosynthesis of Poly(3HB- co-3HP) with Variable Monomer Composition in Recombinant Cupriavidus necator H16. ACS Synthetic Biology, 10(12), 3343-3352. https://doi.org/10.1021/acssynbio.1c00283

Polyhydroxyalkanoates are attractive alternatives to traditional plastics. However, although polyhydroxybutyrate (PHB) is produced in large quantities by Cupriavidus necator H16, its properties are far from ideal for the manufacture of plastic produc... Read More about Biosynthesis of Poly(3HB- co-3HP) with Variable Monomer Composition in Recombinant Cupriavidus necator H16.