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Outputs (18)

Co-assembling living material as an in vitro lung epithelial infection model (2023)
Journal Article
Wu, Y., Romero, M., Robertson, S. N., Fenn, S., Fisher, L., Willingham, I., …Mata, A. (2024). Co-assembling living material as an in vitro lung epithelial infection model. Matter, 7(1), 216-236. https://doi.org/10.1016/j.matt.2023.10.029

Biofilms are robust living 3D materials that play key roles in nature but also cause major problems, such as tolerance to antibiotic treatment. Recreation of these living structures in vitro is critical to understand their biology and develop solutio... Read More about Co-assembling living material as an in vitro lung epithelial infection model.

Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS (2023)
Journal Article
Khateb, H., Hook, A. L., Kern, S., Watts, J. A., Singh, S., Jackson, D., …Alexander, M. R. (2023). Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS. Biointerphases, 18(3), Article 031007. https://doi.org/10.1116/6.0002604

Secondary ion mass spectrometry (SIMS) offers advantages over both liquid extraction mass spectrometry and matrix assisted laser desorption mass spectrometry in that it provides the direct in situ analysis of molecules and has the potential to preser... Read More about Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS.

Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages (2023)
Journal Article
Dave, N., Albiheyri, R. S., Wanford, J. J., Green, L. R., Oldfield, N. J., Turner, D. P., …Bayliss, C. D. (2023). Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages. Microbiology, 169(2), https://doi.org/10.1099/mic.0.001305

Colonization of mucosal tissues by Neisseria meningitidis requires adhesion mediated by the type IV pilus and multiple outer-membrane proteins. Penetration of the mucosa and invasion of epithelial cells are thought to contribute to host persistence a... Read More about Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages.

Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo (2022)
Journal Article
Mastrotto, F., Pirazzini, M., Negro, S., Salama, A., Martinez-Pomares, L., & Mantovani, G. (2022). Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo. Journal of the American Chemical Society, 144(50), 23134–23147. https://doi.org/10.1021/jacs.2c10757

The mannose receptor (CD206) is an endocytic receptor expressed by selected innate immune cells and nonvascular endothelium, which plays a critical role in both homeostasis and pathogen recognition. Although its involvement in the development of seve... Read More about Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo.

Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo (2022)
Journal Article
Mastrotto, F., Pirazzini, M., Negro, S., Salama, A., Martinez-Pomares, L., & Mantovani, G. (2022). Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo. Journal of the American Chemical Society, 144(50), 23134-23147. https://doi.org/10.1021/jacs.2c10757

The mannose receptor (CD206) is an endocytic receptor expressed by selected innate immune cells and nonvascular endothelium, which plays a critical role in both homeostasis and pathogen recognition. Although its involvement in the development of seve... Read More about Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo.

Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function (2021)
Journal Article
Singh, S., Almuhanna, Y., Alshahrani, M. Y., Lowman, D. W., Rice, P. J., Gell, C., …Martinez-Pomares, L. (2021). Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function. npj Biofilms and Microbiomes, 7(1), Article 87. https://doi.org/10.1038/s41522-021-00257-w

Bacterial biofilms represent a challenge to the healthcare system because of their resilience against antimicrobials and immune attack. Biofilms consist of bacterial aggregates embedded in an extracellular polymeric substance (EPS) composed of polysa... Read More about Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function.

Development of dual anti-biofilm and anti-bacterial medical devices (2020)
Journal Article
Burroughs, L., Singh, S., Ashraf, W., Martinez-Pomares, L., Bayston, R., & Hook, A. L. (2020). Development of dual anti-biofilm and anti-bacterial medical devices. Biomaterials Science, 8(14), 3926-3934. https://doi.org/10.1039/d0bm00709a

The rising occurrence of antimicrobial resistance demands new strategies for delivering antibiotics to ensure their effective use. In this study, a multi-functional strategy to address medical device associated infections is explored whereby an anti-... Read More about Development of dual anti-biofilm and anti-bacterial medical devices.

Engulfment, persistance and fate of Bdellovibrio bacteriovorus predators inside human phagocytic cells informs their future therapeutic potential (2019)
Journal Article
Raghunathan, D., Radford, P. M., Gell, C., Negus, D., Moore, C., Till, R., …Tyson, J. (2019). Engulfment, persistance and fate of Bdellovibrio bacteriovorus predators inside human phagocytic cells informs their future therapeutic potential. Scientific Reports, 9, 1-16. https://doi.org/10.1038/s41598-019-40223-3

In assessing the potential of predatory bacteria, such as Bdellovibrio bacteriovorus, to become live therapeutic agents against bacterial infections, it is crucial to understand and quantify Bdellovibrio host cell interactions at a molecular level. H... Read More about Engulfment, persistance and fate of Bdellovibrio bacteriovorus predators inside human phagocytic cells informs their future therapeutic potential.

IFN-γ and IL-17A differentially influence the response of human macrophages and neutrophils to Pseudomonas aeruginosa infection (2018)
Journal Article
Muntaka, S., Almuhanna, Y., Jackson, D., Singh, S., Afryie-Asante, A., Cámara, M., & Martínez-Pomares, L. (2019). IFN-γ and IL-17A differentially influence the response of human macrophages and neutrophils to Pseudomonas aeruginosa infection. Infection and Immunity, 87(2), https://doi.org/10.1128/iai.00814-18

Macrophages are important orchestrators of inflammation during bacterial infection acting both as effector cells and as regulators of neutrophil recruitment and life span. Differently activated macrophage populations with distinct inflammatory and mi... Read More about IFN-γ and IL-17A differentially influence the response of human macrophages and neutrophils to Pseudomonas aeruginosa infection.

Intracellular replication of Streptococcus pneumoniae inside splenic macrophages serves as a reservoir for septicaemia (2018)
Journal Article
Ercoli, G., Fernandes, V. E., Chung, W. Y., Wanford, J. J., Thomson, S., Bayliss, C. D., …Oggioni, M. R. (2018). Intracellular replication of Streptococcus pneumoniae inside splenic macrophages serves as a reservoir for septicaemia. Nature Microbiology, 3, https://doi.org/10.1038/s41564-018-0147-1

Bacterial septicaemia is a major cause of mortality, but its pathogenesis remains poorly understood. In experimental pneumococcal murine intravenous infection, an initial reduction of bacteria in the blood is followed hours later by a fatal septicaem... Read More about Intracellular replication of Streptococcus pneumoniae inside splenic macrophages serves as a reservoir for septicaemia.