Outputs (4)

Development of a novel cell-based, In-Cell Western/ERK assay system for the high-throughput screening of agonists acting on the delta-opioid receptor (2022)
Journal Article
Asghar, J., Latif, L., Alexander, S. P. H., & Kendall, D. A. (2022). Development of a novel cell-based, In-Cell Western/ERK assay system for the high-throughput screening of agonists acting on the delta-opioid receptor. Frontiers in Pharmacology, 13, Article 933356. https://doi.org/10.3389/fphar.2022.933356

Background: Extracellular signal-regulated kinases (ERKs) are important signaling mediators in mammalian cells and, as a result, one of the major areas of research focus. The detection and quantification of ERK phosphorylation as an index of activati... Read More about Development of a novel cell-based, In-Cell Western/ERK assay system for the high-throughput screening of agonists acting on the delta-opioid receptor.

Non‐canonical endogenous expression of voltage‐gated sodium channel NaV1.7 subtype by the TE671 rhabdomyosarcoma cell line (2022)
Journal Article
Aziz, M. Y., Mohammed Latif, L., Ngum, N. M., Aziz, M. Y. A., Latif, M. L., Wall, R. J., …Mellor, I. R. (2022). Non‐canonical endogenous expression of voltage‐gated sodium channel NaV1.7 subtype by the TE671 rhabdomyosarcoma cell line. Journal of Physiology, 600(10), 2499-2513. https://doi.org/10.1113/JP283055

Abstract: The human TE671 cell line was originally used as a model of medulloblastoma but has since been reassigned as rhabdomyosarcoma. Despite the characterised endogenous expression of voltage-sensitive sodium currents in these cells, the specific... Read More about Non‐canonical endogenous expression of voltage‐gated sodium channel NaV1.7 subtype by the TE671 rhabdomyosarcoma cell line.

UDP-sugars activate P2Y 14 receptors to mediate vasoconstriction of the porcine coronary artery (2017)
Journal Article
Abbas, Z. S., Latif, M. L., Dovlatova, N., Fox, S. C., Heptinstall, S., Dunn, W. R., & Ralevic, V. (2018). UDP-sugars activate P2Y 14 receptors to mediate vasoconstriction of the porcine coronary artery. Vascular Pharmacology, 103-105, https://doi.org/10.1016/j.vph.2017.12.063

Aims UDP-sugars can act as extracellular signalling molecules, but relatively little is known about their cardiovascular actions. The P2Y14 receptor is a Gi/o-coupled receptor which is activated by UDP-glucose and related sugar nucleotides. In thi... Read More about UDP-sugars activate P2Y 14 receptors to mediate vasoconstriction of the porcine coronary artery.

Novel vasocontractile role of the P2Y14receptor: characterization of its signalling in porcine isolated pancreatic arteries (2014)
Journal Article
Alsaqati, M., Latif, M., Chan, S., & Ralevic, V. (2014). Novel vasocontractile role of the P2Y14receptor: characterization of its signalling in porcine isolated pancreatic arteries. British Journal of Pharmacology, 171(3), https://doi.org/10.1111/bph.12473

Background and Purpose: The P2Y14 receptor is the newest member of the P2Y receptor family; it is Gi/o protein-coupled and is activated by UDP and selectively by UDP-glucose and MRS2690 (2-thiouridine-5′-diphosphoglucose) (7–10-fold more potent than... Read More about Novel vasocontractile role of the P2Y14receptor: characterization of its signalling in porcine isolated pancreatic arteries.