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Outputs (5)

Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations (2023)
Journal Article
Seys, F. M., Humphreys, C. M., Tomi-Andrino, C., Li, Q., Millat, T., Yang, S., & Minton, N. P. (2023). Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations. Frontiers in Bioengineering and Biotechnology, 11, Article 1211197. https://doi.org/10.3389/fbioe.2023.1211197

Base editors are recent multiplex gene editing tools derived from the Cas9 nuclease of Streptomyces pyogenes. They can target and modify a single nucleotide in the genome without inducing double-strand breaks (DSB) of the DNA helix. As such, they hol... Read More about Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations.

Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum (2022)
Journal Article
Woods, C., Humphreys, C. M., Tomi-Andrino, C., Henstra, A. M., Köpke, M., Simpson, S. D., …Minton, N. P. (2022). Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum. Applied and Environmental Microbiology, 88(7), https://doi.org/10.1128/aem.02479-21

The majority of the genes present in bacterial genomes remain poorly characterized, with up to one-third of those that are protein encoding having no definitive function. Transposon insertion sequencing represents a high-throughput technique that can... Read More about Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum.

Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum (2022)
Journal Article
Piatek, P., Humphreys, C., Raut, M., Wright, P. C., Simpson, S., Köpke, M., …Winzer, K. (2022). Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum. Scientific Reports, 12(1), Article 411. https://doi.org/10.1038/s41598-021-03999-x

Acetogenic bacteria are capable of fermenting CO2 and carbon monoxide containing waste-gases into a range of platform chemicals and fuels. Despite major advances in genetic engineering and improving these biocatalysts, several important physiological... Read More about Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum.

Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system (2019)
Journal Article
Ingle, P., Groothuis, D., Rowe, P., Huang, H., Cockayne, A., Kuehne, S. A., …Minton, N. P. (2019). Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system. Scientific Reports, 9, Article 8123. https://doi.org/10.1038/s41598-019-44458-y

© 2019, The Author(s). Understanding the molecular pathogenesis of Clostridioides difficile has relied on the use of ermB-based mutagens in erythromycin-sensitive strains. However, the repeated subcultures required to isolate sensitive variants can l... Read More about Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system.

Advances in metabolic engineering in the microbial production of fuels and chemicals from C1 gas (2018)
Journal Article
Humphreys, C. M., & Minton, N. P. (in press). Advances in metabolic engineering in the microbial production of fuels and chemicals from C1 gas. Current Opinion in Biotechnology, 50, https://doi.org/10.1016/j.copbio.2017.12.023

The future sustainable production of chemicals and fuels from non-petrochemical sources, while at the same time reducing greenhouse gas (GHG) emissions, represent two of society's greatest challenges. Microbial chassis able to grow on waste carbon mo... Read More about Advances in metabolic engineering in the microbial production of fuels and chemicals from C1 gas.