All Outputs (17)

Towards Development of a Non-Toxigenic Clostridioides difficile Oral Spore Vaccine against Toxigenic C. difficile (2022)
Journal Article
Hughes, J., Aston, C., Kelly, M. L., & Griffin, R. (2022). Towards Development of a Non-Toxigenic Clostridioides difficile Oral Spore Vaccine against Toxigenic C. difficile. Pharmaceutics, 14(5), Article 1086. https://doi.org/10.3390/pharmaceutics14051086

Clostridioides difficile is an opportunistic gut pathogen which causes severe colitis, leading to significant morbidity and mortality due to its toxins, TcdA and TcdB. Two intra-muscular toxoid vaccines entered Phase III trials and strongly induced t... Read More about Towards Development of a Non-Toxigenic Clostridioides difficile Oral Spore Vaccine against Toxigenic C. difficile.

Mimicking native display of cd0873 on liposomes augments its potency as an oral vaccine against clostridioides difficile (2021)
Journal Article
Karyal, C., Palazi, P., Hughes, J., Griffiths, R. C., Persaud, R. R., Tighe, P. J., …Griffin, R. (2021). Mimicking native display of cd0873 on liposomes augments its potency as an oral vaccine against clostridioides difficile. Vaccines, 9(12), Article 1453. https://doi.org/10.3390/vaccines9121453

Mucosal vaccination aims to prevent infection mainly by inducing secretory IgA (sIgA) antibody, which neutralises pathogens and enterotoxins by blocking their attachment to epithelial cells. We previously demonstrated that encapsulated protein antige... Read More about Mimicking native display of cd0873 on liposomes augments its potency as an oral vaccine against clostridioides difficile.

A Multi-Factorial Observational Study on Sequential Fecal Microbiota Transplant in Patients with Medically Refractory Clostridioides difficile Infection (2021)
Journal Article
Monaghan, T. M., Duggal, N. A., Rosati, E., Griffin, R., Hughes, J., Roach, B., …Kao, D. H. (2021). A Multi-Factorial Observational Study on Sequential Fecal Microbiota Transplant in Patients with Medically Refractory Clostridioides difficile Infection. Cells, 10(11), Article 3234. https://doi.org/10.3390/cells10113234

Fecal microbiota transplantation (FMT) is highly effective in recurrent Clostridioides difficile infection (CDI); increasing evidence supports FMT in severe or fulminant Clostridioides difficile infection (SFCDI). However, the multifactorial mechanis... Read More about A Multi-Factorial Observational Study on Sequential Fecal Microbiota Transplant in Patients with Medically Refractory Clostridioides difficile Infection.

Colonisation factor cd0873, an attractive oral vaccine candidate against clostridioides difficile (2021)
Journal Article
Karyal, C., Hughes, J., Kelly, M. L., Cockayne, A., Luckett, J. C., Kaye, P. V., …Griffin, R. (2021). Colonisation factor cd0873, an attractive oral vaccine candidate against clostridioides difficile. Microorganisms, 9(2), Article 306. https://doi.org/10.3390/microorganisms9020306

Clostridioides difficile is the main cause of health-care-associated infectious diarrhoea. Toxins, TcdA and TcdB, secreted by this bacterium damage colonic epithelial cells and in severe cases this culminates in pseudomembranous colitis, toxic megaco... Read More about Colonisation factor cd0873, an attractive oral vaccine candidate against clostridioides difficile.

Variant Signal Peptides of Vaccine Antigen, FHbp, Impair Processing Affecting Surface Localization and Antibody-Mediated Killing in Most Meningococcal Isolates (2019)
Journal Article
da Silva, R. A., Karlyshev, A. V., Oldfield, N. J., Wooldridge, K. G., Bayliss, C. D., Ryan, A., & Griffin, R. (2019). Variant Signal Peptides of Vaccine Antigen, FHbp, Impair Processing Affecting Surface Localization and Antibody-Mediated Killing in Most Meningococcal Isolates. Frontiers in Microbiology, 10, https://doi.org/10.3389/fmicb.2019.02847

© Copyright © 2019 da Silva, Karlyshev, Oldfield, Wooldridge, Bayliss, Ryan and Griffin. Meningococcal lipoprotein, Factor H binding protein (FHbp), is the sole antigen of the Trumenba vaccine (Pfizer) and one of four antigens of the Bexsero vaccine... Read More about Variant Signal Peptides of Vaccine Antigen, FHbp, Impair Processing Affecting Surface Localization and Antibody-Mediated Killing in Most Meningococcal Isolates.

Exposing hidden putative lipoproteins in Clostridium difficile (2017)
Presentation / Conference
Griffin, R., & Minton, N. P. (2017, August). Exposing hidden putative lipoproteins in Clostridium difficile. Poster presented at ClostPath 10th International Conference on the Molecular Biology and Pathogenesis of the Clostrida, Ann Arbor, Michigan, USA

The role of apolipoprotein N-acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target (2016)
Journal Article
da Silva, R., Churchward, C., Karlyshev, A., Eleftheriadou, O., Snabaitis, A., Longman, M., …Griffin, R. (2017). The role of apolipoprotein N-acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target. British Journal of Pharmacology, 174(14), 2247-2260. https://doi.org/10.1111/bph.13660

BACKGROUND AND PURPOSE The level of cell surface expression of the meningococcal vaccine antigen, Factor H binding protein (FHbp) varies between and within strains and this limits the breadth of strains that can be targeted by FHbp-based vaccines.... Read More about The role of apolipoprotein N-acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target.

Insight into proteomic investigations of Neisseria meningitidis serogroup C strain L91543 from analysis of its genome sequence (2015)
Journal Article
Karlyshev, A., Snyder, L. A., McFadden, J., & Griffin, R. (2015). Insight into proteomic investigations of Neisseria meningitidis serogroup C strain L91543 from analysis of its genome sequence. FEMS Microbiology Letters, 362(9), Article fnv055. https://doi.org/10.1093/femsle/fnv055

Here, we describe the draft sequence of a virulent isolate of Neisseria meningitidis strain L91543, belonging to serogroup C. The findings from previous proteomic and metabolomic studies of this strain can now be further interpreted with genomic anal... Read More about Insight into proteomic investigations of Neisseria meningitidis serogroup C strain L91543 from analysis of its genome sequence.

The future of tuberculosis vaccinology (2009)
Book Chapter
Thole, J., Griffin, R., & Young, D. (2009). The future of tuberculosis vaccinology. In M. C. Raviglione (Ed.), Tuberculosis: the essentials. (4th edition). Boca Raton, FL: CRC Press. https://doi.org/10.3109/9781420090239

© 2006 by Taylor and Francis Group, LLC. The outcome of infection with Mycobacterium tuberculosis is crucially dependent on the immune response of the host. Most individuals mount a response that is sufficient to prevent progression to disease but ma... Read More about The future of tuberculosis vaccinology.

Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages (2006)
Journal Article
Rosas-Magallanes, V., Stadthagen-Gomez, G., Rauzier, J., Barreiro, L. B., Tailleux, L., Boudou, F., …Neyrolles, O. (2007). Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages. Infection and Immunity, 75(1), 504-507. https://doi.org/10.1128/IAI.00058-06

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-enco... Read More about Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages.

The future of tuberculosis vaccinology (2006)
Book Chapter
Griffin, R., & Young, D. (2006). The future of tuberculosis vaccinology. In M. C. Raviglione (Ed.), Reichman and Hershfield's tuberculosis : a comprehensive, international approach (1153-1168). (3rd ed.). Informa Healthcare

Comparative investigation of the pathogenicity of three Mycobacterium tuberculosis mutants defective in the synthesis of p-hydroxybenzoic acid derivatives (2006)
Journal Article
Stadthagen, G., Jackson, M., Charles, P., Boudoua, F., Barilonea, N., Huerree, M., …Griffin, R. (in press). Comparative investigation of the pathogenicity of three Mycobacterium tuberculosis mutants defective in the synthesis of p-hydroxybenzoic acid derivatives. Microbes and Infection, 8(8), https://doi.org/10.1016/j.micinf.2006.04.008

p-Hydroxybenzoic acid derivatives (p-HBADs) are glycoconjugates secreted by all Mycobacterium tuberculosis isolates whose contribution to pathogenicity remains to be determined. The pathogenicity of three transposon mutants of M. tuberculosis deficie... Read More about Comparative investigation of the pathogenicity of three Mycobacterium tuberculosis mutants defective in the synthesis of p-hydroxybenzoic acid derivatives.

p-Hydroxybenzoic acid synthesis in mycobacterium tuberculosis (2005)
Journal Article
Stadthagen, G., Kordula´kova, J., Griffin, R., Constant, P., Bottova, I., Barilone, N., …Jackson, M. (2005). p-Hydroxybenzoic acid synthesis in mycobacterium tuberculosis. Journal of Biological Chemistry, 280(49), https://doi.org/10.1074/jbc.M508332200

Glycosylated p-hydroxybenzoic acid methyl esters and structurally related phenolphthiocerol glycolipids are important virulence factors of Mycobacterium tuberculosis. Although both types of molecules are thought to be derived from p-hydroxybenzoic ac... Read More about p-Hydroxybenzoic acid synthesis in mycobacterium tuberculosis.

Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival (2005)
Journal Article
Griffin, R., Bayliss, C. D., Herbert, M. A., Cox, A. D., Makepeace, K., Richards, J., …Moxon, E. R. (2005). Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival. Infection and Immunity, 73(10), https://doi.org/10.1128/IAI.73.10.7022-7026.2005

Digalactoside (gal-1-4 gal) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expressio... Read More about Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival.

Elucidation of the monoclonal antibody 5G8-reactive, virulence-associated lipopolysaccharide epitope of haemophilus influenzae and its role in bacterial resistance to complement-mediated killing (2005)
Journal Article
Griffin, R., Cox, A. D., Makepeace, K., Richards, J., Moxon, E. R., & Hood, D. W. (2005). Elucidation of the monoclonal antibody 5G8-reactive, virulence-associated lipopolysaccharide epitope of haemophilus influenzae and its role in bacterial resistance to complement-mediated killing. Infection and Immunity, 73(4), https://doi.org/10.1128/IAI.73.4.2213-2221.2005

The phase-variable locus lex2 is required for expression of a Haemophilus influenzae lipopolysaccharide (LPS) epitope of previously unknown structure. This epitope, which is reactive with monoclonal antibody (MAb) 5G8, has been associated with virule... Read More about Elucidation of the monoclonal antibody 5G8-reactive, virulence-associated lipopolysaccharide epitope of haemophilus influenzae and its role in bacterial resistance to complement-mediated killing.

The role of lex2 in lipopolysaccharide biosynthesis in Haemophilus influenzae strains RM7004 and RM153 (2003)
Journal Article
Griffin, R., Cox, A. D., Makepeace, K., Richards, J. C., Moxon, E. R., & Hood, D. W. (in press). The role of lex2 in lipopolysaccharide biosynthesis in Haemophilus influenzae strains RM7004 and RM153. Microbiology, 149(11), https://doi.org/10.1099/mic.0.26387-0

The locus lex2, comprising lex2A and lex2B, contributes to the phase-variable expression of lipopolysaccharide (LPS) of Haemophilus influenzae and was found to be present in 74 % of strains investigated. lex2A contains 59-GCAA repeats which vary in n... Read More about The role of lex2 in lipopolysaccharide biosynthesis in Haemophilus influenzae strains RM7004 and RM153.

The pathogenesis of disease due to type b Haemophilus influenzae (2003)
Book Chapter
Aubrey, R., & Tang, C. (2003). The pathogenesis of disease due to type b Haemophilus influenzae. In M. A. Herbert, D. W. Hood, & E. R. Moxon (Eds.), Haemophilus influenzae protocols. Humana Press Inc. https://doi.org/10.1385/1-59259-321-6%3A29

Haemophilus influenzae is a Gram-negative bacterium that was first described by Pfeiffer in 1892 (1). This ubiquitous, human-specific organism was originally thought to be the etiologic agent of “influenza.” However, H. influenzae was not consistentl... Read More about The pathogenesis of disease due to type b Haemophilus influenzae.