All Outputs (11)

A diterpene synthase from the sandfly Lutzomyia longipalpis produces the pheromone sobralene (2024)
Journal Article
Ducker, C., Baines, C., Guy, J., Euzébio Goulart Santana, A., Pickett, J. A., & Oldham, N. J. (2024). A diterpene synthase from the sandfly Lutzomyia longipalpis produces the pheromone sobralene. Proceedings of the National Academy of Sciences, 121(12), Article e2322453121. https://doi.org/10.1073/pnas.2322453121

The phlebotomine sandfly, Lutzomyia longipalpis, a major vector of the Leishmania parasite, uses terpene pheromones to attract conspecifics for mating. Examination of the L. longipalpis genome revealed a putative terpene synthase (TPS), which—upon he... Read More about A diterpene synthase from the sandfly Lutzomyia longipalpis produces the pheromone sobralene.

Characterisation of geranylgeranyl diphosphate synthase from the sandfly Lutzomyia longipalpis (2023)
Journal Article
Ducker, C., French, S., Pathak, M., Taylor, H., Sainter, A., Askem, W., …Oldham, N. J. (2023). Characterisation of geranylgeranyl diphosphate synthase from the sandfly Lutzomyia longipalpis. Insect Biochemistry and Molecular Biology, 161, Article 104001. https://doi.org/10.1016/j.ibmb.2023.104001

Leishmaniasis is a debilitating and often fatal neglected tropical disease. Males from sub-populations of the Leishmania-harbouring sandfly, Lutzomyia longipalpis, produce the diterpene sex and aggregation pheromone, sobralene, for which geranylgeran... Read More about Characterisation of geranylgeranyl diphosphate synthase from the sandfly Lutzomyia longipalpis.

The Small Molecule Antagonist KCI807 Disrupts Association of the Amino-Terminal Domain of the Androgen Receptor with ELK1 by Modulating the Adjacent DNA Binding Domain (2023)
Journal Article
Soave, C., Ducker, C., Islam, N., Kim, S., Yurgelevic, S., Nicely, N. I., …Ratnam, M. (2023). The Small Molecule Antagonist KCI807 Disrupts Association of the Amino-Terminal Domain of the Androgen Receptor with ELK1 by Modulating the Adjacent DNA Binding Domain. Molecular Pharmacology, 103(4), 211-220. https://doi.org/10.1124/molpharm.122.000589

The androgen receptor (AR) is a crucial coactivator of ELK1 for prostate cancer (PCa) growth, associating with ELK1 through two peptide segments (358-457 and 514-557) within the amino-terminal domain (NTD) of AR. The small-molecule antagonist 5-hydro... Read More about The Small Molecule Antagonist KCI807 Disrupts Association of the Amino-Terminal Domain of the Androgen Receptor with ELK1 by Modulating the Adjacent DNA Binding Domain.

Comparative analysis of protein expression systems and PTM landscape in the study of transcription factor ELK-1 (2022)
Journal Article
Ducker, C., Ratnam, M., Shaw, P. E., & Layfield, R. (2023). Comparative analysis of protein expression systems and PTM landscape in the study of transcription factor ELK-1. Protein Expression and Purification, 203, Article 106216. https://doi.org/10.1016/j.pep.2022.106216

Post-translational modifications (PTMs) are important for protein folding and activity, and the ability to recreate physiologically relevant PTM profiles on recombinantly-expressed proteins is vital for meaningful functional analysis. The ETS transcr... Read More about Comparative analysis of protein expression systems and PTM landscape in the study of transcription factor ELK-1.

Identification of ELK1 interacting peptide segments in the androgen receptor (2022)
Journal Article
Ducker, C., Kim, S., Strahl, T., Rosati, R., Huang, Y., Shaw, P. E., & Ratnam, M. (2022). Identification of ELK1 interacting peptide segments in the androgen receptor. Biochemical Journal, 479(14), 1519-1531. https://doi.org/10.1042/bcj20220297

Prostate cancer (PCa) growth requires tethering of the androgen receptor (AR) to chromatin by the ETS domain transcription factor ELK1 to coactivate critical cell proliferation genes. Disruption of the ELK1–AR complex is a validated potential means o... Read More about Identification of ELK1 interacting peptide segments in the androgen receptor.

Ubiquitin-Mediated Control of ETS Transcription Factors: Roles in Cancer and Development (2021)
Journal Article
Ducker, C., & Shaw, P. E. (2021). Ubiquitin-Mediated Control of ETS Transcription Factors: Roles in Cancer and Development. International Journal of Molecular Sciences, 22(10), Article 5119. https://doi.org/10.3390/ijms22105119

Genome expansion, whole genome and gene duplication events during metazoan evolution produced an extensive family of ETS genes whose members express transcription factors with a conserved winged helix-turn-helix DNA-binding domain. Unravelling their... Read More about Ubiquitin-Mediated Control of ETS Transcription Factors: Roles in Cancer and Development.

ELK-1 ubiquitination status and transcriptional activity are modulated independently of F-Box protein FBXO25 (2021)
Journal Article
Quintero-Barceinas, R. S., Gehringer, F., Ducker, C., Saxton, J., & Shaw, P. E. (2021). ELK-1 ubiquitination status and transcriptional activity are modulated independently of F-Box protein FBXO25. Journal of Biological Chemistry, 296, Article 100214. https://doi.org/10.1074/jbc.ra120.014616

The mitogen-responsive, ETS-domain transcription factor ELK-1 stimulates the expression of immediate early genes at the onset of the cell cycle and participates in early developmental programming. ELK-1 is subject to multiple levels of posttranslatio... Read More about ELK-1 ubiquitination status and transcriptional activity are modulated independently of F-Box protein FBXO25.

USP17-mediated de-ubiquitination and cancer: Clients cluster around the cell cycle (2020)
Journal Article
Ducker, C., & Shaw, P. E. (2021). USP17-mediated de-ubiquitination and cancer: Clients cluster around the cell cycle. International Journal of Biochemistry and Cell Biology, 130, Article 105886. https://doi.org/10.1016/j.biocel.2020.105886

Eukaryotic cells perform a range of complex processes, some essential for life, others specific to cell type, all of which are governed by post-translational modifications of proteins. Among the repertoire of dynamic protein modifications, ubiquitina... Read More about USP17-mediated de-ubiquitination and cancer: Clients cluster around the cell cycle.

De-ubiquitination of ELK-1 by USP17 potentiates mitogenic gene expression and cell proliferation (2019)
Journal Article
Ducker, C., Chow, L., Saxton, J., Handwerger, J., McGregor, A., Strahl, T., …Shaw, P. E. (2019). De-ubiquitination of ELK-1 by USP17 potentiates mitogenic gene expression and cell proliferation. Nucleic Acids Research, 47(9), 4495-4508. https://doi.org/10.1093/nar/gkz166

ELK-1 is a transcription factor involved in ERK-induced cellular proliferation. Here we show that its transcriptional activity is modulated by ubiquitination at lysine 35 (K35). The level of ubiquitinated ELK-1 rises in mitogen-deprived cells and fal... Read More about De-ubiquitination of ELK-1 by USP17 potentiates mitogenic gene expression and cell proliferation.

Strategy for tumor selective disruption of androgen receptor function in the spectrum of prostate cancer (2018)
Journal Article
Rosati, R., Polin, L. A., Ducker, C., Li, J., Bao, X., Selvakumar, D., …Ratnam, M. (2018). Strategy for tumor selective disruption of androgen receptor function in the spectrum of prostate cancer. Clinical Cancer Research, https://doi.org/10.1158/1078-0432.ccr-18-0982

Purpose: Testosterone suppression in prostate cancer (PC) is limited by serious side effects and resistance via restoration of androgen receptor (AR) functionality. ELK1 is required for ARdependent growth in various hormone-dependent and castration r... Read More about Strategy for tumor selective disruption of androgen receptor function in the spectrum of prostate cancer.

Stepwise evolution of Elk-1 in early deuterostomes (2016)
Journal Article
Saxton, J., Ferjentsik, Z., Ducker, C., & Shaw, P. E. (in press). Stepwise evolution of Elk-1 in early deuterostomes. FEBS Journal, 283(6), https://doi.org/10.1111/febs.13607

Metazoans have multiple ETS paralogues with overlapping or indiscriminate biological functions. Elk- 1, one of three mammalian Ternary Complex Factors (TCFs), is a well-conserved, ETS domain-containing transcriptional regulator of mitogen-responsive... Read More about Stepwise evolution of Elk-1 in early deuterostomes.