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All Outputs (8)

Antibody-based sex determination of human skeletal remains (2023)
Journal Article
Shaw, B., Foggin, S., Hamilton-Stanley, P., Barlow, A., Pickard, C., Fibiger, L., …Layfield, R. (2023). Antibody-based sex determination of human skeletal remains. iScience, 26(11), Article 108191. https://doi.org/10.1016/j.isci.2023.108191

Assignment of biological sex to skeletal remains is critical in the accurate reconstruction of the past. Analysis of sex-chromosome encoded AMELX and AMELY peptides from the enamel protein amelogenin underpins a minimally destructive mass spectrometr... Read More about Antibody-based sex determination of human skeletal remains.

Osteological, multi-isotope and proteomic analysis of poorly-preserved human remains from a Dutch East India Company burial ground in South Africa (2023)
Journal Article
Olszewski, J., Hall, R. A., Kootker, L. M., Oldham, N. J., Layfield, R., Shaw, B., …Schrader, S. A. (2023). Osteological, multi-isotope and proteomic analysis of poorly-preserved human remains from a Dutch East India Company burial ground in South Africa. Scientific Reports, 13, Article 14666. https://doi.org/10.1038/s41598-023-41503-9

Skeletal remains discovered in Simon’s Town, South Africa, were hypothesised as being associated with a former Dutch East India Company (VOC) hospital. We report a novel combined osteological and biochemical approach to these poorly-preserved remains... Read More about Osteological, multi-isotope and proteomic analysis of poorly-preserved human remains from a Dutch East India Company burial ground in South Africa.

Preservation of whole antibodies within ancient teeth (2023)
Journal Article
Shaw, B., McDonnell, T., Radley, E., Thomas, B., Smith, L., Davenport, C. A. L., …Layfield, R. (2023). Preservation of whole antibodies within ancient teeth. iScience, 26(9), Article 107575. https://doi.org/10.1016/j.isci.2023.107575

Archaeological remains can preserve some proteins into deep time, offering remarkable opportunities for probing past events in human history. Recovering functional proteins from skeletal tissues could uncover a molecular memory related to the life-hi... Read More about Preservation of whole antibodies within ancient teeth.

Molecular insights into an ancient form of Paget's disease of bone (2019)
Journal Article
Shaw, B., Burrell, C. L., Green, D., Navarro-Martinez, A., Scott, D., Daroszewska, A., …Layfield, R. (2019). Molecular insights into an ancient form of Paget's disease of bone. Proceedings of the National Academy of Sciences, 116(21), 10463-10472. https://doi.org/10.1073/pnas.1820556116

We identify an ancient and atypical form of Paget’s disease of bone (PDB) in a collection of medieval skeletons exhibiting unusually extensive pathological changes, high disease prevalence, and low age-at-death estimations. Proteomic analysis of anci... Read More about Molecular insights into an ancient form of Paget's disease of bone.

ALS-FTLD associated mutations of SQSTM1 impact on Keap1-Nrf2 signalling (2016)
Journal Article
Goode, A., Rea, S., Sultana, M., Shaw, B., Searle, M. S., & Layfield, R. (2016). ALS-FTLD associated mutations of SQSTM1 impact on Keap1-Nrf2 signalling. Molecular and Cellular Neuroscience, 76, https://doi.org/10.1016/j.mcn.2016.08.004

The transcription factor Nrf2 and its repressor protein Keap1 play key roles in the regulation of antioxidant stress responses and both Keap1-Nrf2 signalling and oxidative stress have been implicated in the pathogenesis of the ALS-FTLD spectrum of ne... Read More about ALS-FTLD associated mutations of SQSTM1 impact on Keap1-Nrf2 signalling.

Defective recognition of LC3B by mutant SQSTM1/p62 implicates impairment of autophagy as a pathogenic mechanism in ALS-FTLD (2016)
Journal Article
Goode, A., Butler, K., Long, J., Cavey, J., Scott, D., Shaw, B., …Layfield, R. (2016). Defective recognition of LC3B by mutant SQSTM1/p62 implicates impairment of autophagy as a pathogenic mechanism in ALS-FTLD. Autophagy, 12(7), 1094-1104. https://doi.org/10.1080/15548627.2016.1170257

Growing evidence implicates impairment of autophagy as a candidate pathogenic mechanism in the spectrum of neurodegenerative disorders which includes amyotrophic lateral sclerosis and frontotemporal lobar degeneration (ALS-FTLD). SQSTM1, which encode... Read More about Defective recognition of LC3B by mutant SQSTM1/p62 implicates impairment of autophagy as a pathogenic mechanism in ALS-FTLD.

Paget disease of bone-associated UBA domain mutations of SQSTM1 exert distinct effects on protein structure and function (2014)
Journal Article
Goode, A., Long, J. E., Shaw, B., Ralston, S. H., Visconti, M. R., Gianfrancesco, F., …Layfield, R. (2014). Paget disease of bone-associated UBA domain mutations of SQSTM1 exert distinct effects on protein structure and function. BBA - Molecular Basis of Disease, 1842(7), 992-1000. https://doi.org/10.1016/j.bbadis.2014.03.006

SQSTM1 mutations are common in patients with Paget disease of bone (PDB), with most affecting the C-terminal ubiquitin-associated (UBA) domain of the SQSTM1 protein. We performed structural and functional analyses of two UBA domain mutations, an I424... Read More about Paget disease of bone-associated UBA domain mutations of SQSTM1 exert distinct effects on protein structure and function.

Knockdown of alpha myosin heavy chain disrupts the cytoskeleton and leads to multiple defects during chick cardiogenesis (2009)
Journal Article
Rutland, C., Warner, L., Thorpe, A., Alibhai, A., Robinson, T., Shaw, B., …Loughna, S. (2009). Knockdown of alpha myosin heavy chain disrupts the cytoskeleton and leads to multiple defects during chick cardiogenesis. Journal of Anatomy, 214(6), 905-915. https://doi.org/10.1111/j.1469-7580.2009.01079.x

Atrial septal defects are a common congenital heart defect in humans. Although mutations in different genes are now frequently being described, little is known about the processes and mechanisms behind the early stages of atrial septal development. B... Read More about Knockdown of alpha myosin heavy chain disrupts the cytoskeleton and leads to multiple defects during chick cardiogenesis.