Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose

Abidin, Munira Z; Kourmentza, Constantina; Karatzas, Andreas K; Niranjan, Keshavan

Abstract

BACKGROUND: N,N′-diacetylchitobiose (GlcNAc2) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre-treatment process using steam under pressure in an autoclave was employed to facilitate subsequent enzymatic hydrolysis of chitin with chitinase from Streptomyces griseus. RESULTS: Pre-treatment of chitin with 0.05mol L−1 sodium acetate buffer (pH =6.0) at 121 °C for 60 min, followed by enzymatic hydrolysis involving 24 h incubation, were found to be the best conditions for producing GlcNAc2. The GlcNAc2 thus obtained was tested regarding its antimicrobial activity against Gram-negative and Gram-positive strains and showed minimum inhibitory concentrations at 5 and 10% (w/v) against Escherichia coli K-12 and Listeria monocytogenes 10403S, respectively. CONCLUSIONS: The extent of swelling and crystallite size of chitin increased with pre-treatment residence time, and enhanced the rate of subsequent hydrolysis using chitinase. © 2019 Society of Chemical Industry.

Citation

Abidin, M. Z., Kourmentza, C., Karatzas, A. K., & Niranjan, K. (2019). Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose. Journal of Chemical Technology and Biotechnology, 94(8), 2529-2536. https://doi.org/10.1002/jctb.6043

• Journal Article Type: Article
• Acceptance Date: Apr 18, 2019
• Online Publication Date: May 16, 2019
• Publication Date: 2019-08
• Deposit Date: Jul 27, 2020
• Journal: Journal of Chemical Technology and Biotechnology
• Print ISSN: 0268-2575
• Electronic ISSN: 1097-4660
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 94
• Issue: 8
• Pages: 2529-2536
• DOI: https://doi.org/10.1002/jctb.6043
• Keywords: Biotechnology; Fuel Technology; Inorganic Chemistry; Renewable Energy, Sustainability and the Environment; Organic Chemistry; Waste Management and Disposal; Pollution; General Chemical Engineering
• Public URL: https://nottingham-repository.worktribe.com/output/3481478
• Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.6043