@article { ,
title = {Evaluation of diffusion coefficients by means of an approximate steady-state condition in sedimentation velocity distributions},
abstract = {This investigation examined the feasibility of manipulating the rotor speed in sedimentation velocity experiments to spontaneously generate an approximate steady-state condition where the extent of diffusional spreading is matched exactly by the boundary sharpening arising from negative s–c dependence. Simulated sedimentation velocity distributions based on the sedimentation characteristics for a purified mucin preparation were used to illustrate a simple procedure for determining the diffusion coefficient from such steady-state distributions in situations where the concentration dependence of the sedimentation coefficient, s = s0/(1 + Kc), was quantified in terms of the limiting sedimentation coefficient as c → 0 (s0) and the concentration coefficient (K). Those simulations established that spontaneous generation of the approximate steady state could well be a feature of sedimentation velocity distributions for many unstructured polymer systems because the requirement that Kcoω2s0/D be between 46 and 183 cm−2 is not unduly restrictive. Although spontaneous generation of the approximate steady state is also a theoretical prediction for structured macromolecular solutes exhibiting linear concentration dependence of the sedimentation coefficient, s = s0(1 − kc), the required value of k is far too large for any practical advantage to be taken of this approach with globular proteins.},
doi = {10.1016/j.ab.2015.08.017},
eissn = {1096-0309},
issn = {0003-2697},
journal = {Analytical Biochemistry},
note = {Needs AAM with CCANCND.},
publicationstatus = {Published},
publisher = {Elsevier},
url = {https://nottingham-repository.worktribe.com/output/765055},
volume = {490},
keyword = {Diffusion coefficient, Sedimentation velocity, Ultracentrifugation},
year = {2015},
author = {Scott, David J. and Harding, Stephen E. and Winzor, Donald J.}
}