@article { , title = {PU.1 is a major transcriptional activator of the tumour suppressor gene LIMD1}, abstract = {LIMD1 is a tumour suppressor gene (TSG) down regulated in ?80\% of lung cancers with loss also demonstrated in breast and head and neck squamous cell carcinomas. LIMD1 is also a candidate TSG in childhood acute lymphoblastic leukaemia. Mechanistically, LIMD1 interacts with pRB, repressing E2F?driven transcription as well as being a critical component of microRNA?mediated gene silencing. In this study we show a CpG island within the LIMD1 promoter contains a conserved binding motif for the transcription factor PU.1. Mutation of the PU.1 consensus reduced promoter driven transcription by 90\%. ChIP and EMSA analysis demonstrated that PU.1 specifically binds to the LIMD1 promoter. siRNA depletion of PU.1 significantly reduced endogenous LIMD1 expression, demonstrating that PU.1 is a major transcriptional activator of LIMD1.}, doi = {10.1016/j.febslet.2011.03.013}, eissn = {1873-3468}, issn = {0014-5793}, issue = {7}, journal = {FEBS Letters}, pages = {1089-1096}, publicationstatus = {Published}, publisher = {Wiley}, url = {https://nottingham-repository.worktribe.com/output/1376955}, volume = {585}, year = {2011}, author = {Foxler, Daniel E. and James, Victoria and Shelton, Samuel J. and de A. Vallim, Thomas Q. and Shaw, Peter E. and Sharp, Tyson V.} }