@article { , title = {Synthesis, Biological Evaluation of Fluorescent 23-Hydroxybetulinic Acid Probes, and Their Cellular Localization Studies}, abstract = {© 2018 American Chemical Society. 23-Hydroxybetulinic acid (23-HBA) is a complex lupane triterpenoid, which has attracted increasing attention as an anticancer agent. However, its detailed mechanism of anticancer action remains elusive so far. To reveal its anticancer mode of action, a series of fluorescent 23-HBA derivatives conjugated with coumarin dyes were designed, synthesized, and evaluated for their antiproliferative activities. Subcellular localization and uptake profile studies of representative fluorescent 23-HBA probe 26c were performed in B16F10 cells, and the results suggested that probe 26c was rapidly taken up into B10F10 cells in a dose-dependent manner and mitochondrion was the main site of its accumulation. Further mode of action studies implied that the mitochondrial pathway was involved in 23-HBA-mediated apoptosis. Together, our results provided new clues for revealing the molecular mechanism of natural product 23-HBA for its further development into an antitumor agent.}, doi = {10.1021/acsmedchemlett.8b00321}, eissn = {1948-5875}, issn = {1948-5875}, issue = {10}, journal = {ACS Medicinal Chemistry Letters}, pages = {1030-1034}, publicationstatus = {Published}, publisher = {American Chemical Society}, url = {https://nottingham-repository.worktribe.com/output/1370824}, volume = {9}, year = {2018}, author = {Yao, Hong and Wei, Guoxiang and Liu, Yanpeng and Yao, Hequan and Zhu, Zheying and Ye, Wencai and Wu, Xiaoming and Xu, Jinyi and Xu, Shengtao} }