@article { , title = {Endothelin-converting enzyme 1 and ?-arrestins exert spatiotemporal control of substance P-induced inflammatory signals}, abstract = {Although the intracellular trafficking of G protein-coupled receptors controls specific signaling events, it is unclear how the spatiotemporal control of signaling contributes to complex pathophysiological processes such as inflammation. By using bioluminescence resonance energy transfer and superresolution microscopy, we found that substance P (SP) induces the association of the neurokinin 1 receptor(NK1R) with two classes of proteins that regulate SP signaling from plasma and endosomal membranes: the scaffolding proteins β-arrestin (βARRs) 1 and 2 and thetransmembrane metallopeptidases ECE-1c and ECE-1d. In HEK293 cells and non-transformed human colonocytes, we observed that G protein-coupled receptor kinase 2 and βARR1/2 terminate plasma membrane Ca2+ signaling and initiate receptor trafficking to endosomes that is necessary for sustained activation of ERKs in the nucleus. βARRs deliver the SP-NK1R endosomes, where ECE-1 associates with the complex, degrades SP, and allows the NK1R, freed from βARRs, to recycle. Thus, both ECE-1 and βARRs mediate the resensitization of NK1R Ca2+ signaling at the plasma membrane. Sustained exposure of colonocytes to SP activates NF-κB and stimulates IL-8 secretion. This proinflammatory signaling is unaffected by inhibition of the endosomal ERK pathway but is suppressed by ECE-1 inhibition or βARR2 knockdown. Inhibition of protein phosphatase 2A, which also contributes to sustained NK1R signaling at the plasma membrane, similarly attenuates IL-8 secretion. Thus, the primary function of βARRs and ECE-1in SP-dependent inflammatory signaling is to promote resensitization, which allows the sustained NK1R signaling from the plasma membrane that drives inflammation. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.}, doi = {10.1074/jbc.M114.578179}, eissn = {1083-351X}, issn = {0021-9258}, issue = {29}, journal = {Journal of Biological Chemistry}, note = {Cited By :9; Export Date: 27 November 2018; CODEN: JBCHA; Correspondence Address: Bunnett, N.W.; Monash Institute of Pharmaceutical Sciences, 381 Royal Parade, Parkville, VIC 3052, Australia; email: Nigel.Bunnett@Monash.edu}, pages = {20283-20294}, publicationstatus = {Published}, publisher = {American Society for Biochemistry and Molecular Biology}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84904489168\&doi=10.1074\%2fjbc.M114.578179\&partnerID=40\&md5=f1d1920c44ceb348356d8ea9c759d3fd}, volume = {289}, keyword = {Cell membranes, Enzyme activity, Pathology, Physiology, Proteins, Scaffolds, Scaffolds (biology), Signaling, Bioluminescence resonance energy transfer, Endothelin-converting enzymes, G protein coupled receptors, G protein-coupled receptor kinase 2, Inflam}, year = {2024}, author = {Jensen, Dane D. and Halls, Michelle L. and Murphy, Jane E. and Canals, Meritxell and Cattaruzza, Fiore and Poole, Daniel P. and Lieu, Tina Marie and Koon, H.-W. and Pothoulakis, Charalabos and Bunnett, Nigel W.} }