@article { , title = {Patterns of genome evolution that have accompanied host adaptation in Salmonella}, abstract = {Many bacterial pathogens are specialized, infecting one or few hosts, and this is often associated with more acute disease presentation. Specific genomes show markers of this specialization, which often reflect a balance between gene acquisition and functional gene loss. Within Salmonella enterica subspecies enterica, a single lineage exists that includes human and animal pathogens adapted to cause infection in different hosts, including S. enterica serovar Enteritidis (multiple hosts), S. Gallinarum (birds), and S. Dublin (cattle). This provides an excellent evolutionary context in which differences between these pathogen genomes can be related to host range. Genome sequences were obtained from ∼60 isolates selected to represent the known diversity of this lineage. Examination and comparison of the clades within the phylogeny of this lineage revealed signs of host restriction as well as evolutionary events that mark a path to host generalism. We have identified the nature and order of events for both evolutionary trajectories. The impact of functional gene loss was predicted based upon position within metabolic pathways and confirmed with phenotyping assays. The structure of S. Enteritidis is more complex than previously known, as a second clade of S. Enteritidis was revealed that is distinct from those commonly seen to cause disease in humans or animals, and that is more closely related to S. Gallinarum. Isolates from this second clade were tested in a chick model of infection and exhibited a reduced colonization phenotype, which we postulate represents an intermediate stage in pathogen–host adaptation.}, doi = {10.1073/pnas.1416707112}, eissn = {1091-6490}, issn = {0027-8424}, issue = {3}, journal = {Proceedings of the National Academy of Sciences}, note = {Deposited on behalf of leaver.}, pages = {863-868}, publicationstatus = {Published}, publisher = {National Academy of Sciences}, url = {https://nottingham-repository.worktribe.com/output/1103987}, volume = {112}, year = {2015}, author = {Langridge, Gemma C. and Fookes, Maria and Connor, Thomas R. and Feltwell, Theresa and Feasey, Nicholas and Parsons, Bryony N. and Seth-Smith, Helena M.B. and Barquist, Lars and Stedman, Anna and Humphrey, Tom and Wigley, Paul and Peters, Sarah E. and Maskell, Duncan J. and Corander, Jukka and Chabalgoity, Jose A. and Barrow, Paul and Parkhill, Julian and Dougan, Gordon and Thomson, Nicholas R.} }